Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,211	T→G	L371V (TTA→GTA)	ubiF →	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,211	0	T	G	100.0%	76.5 / NA	22	L371V (TTA→GTA)	ubiF	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/8);  total (14/8)

GCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATT  >  NC_000913/696135‑696288                                                                             |                                                                              gCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAAt                                                                           >  1:147911/1‑81 (MQ=255)    aaGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATggc                                                                        <  1:147869/81‑1 (MQ=255)     aGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATggcg                                                                       <  1:147870/81‑1 (MQ=255)        ggTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGgcggcg                                                                    >  1:147904/1‑81 (MQ=255)        ggTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGgcggcg                                                                    >  1:147923/1‑81 (MQ=255)          tATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGgcggcg                                                                    >  2:147905/1‑79 (MQ=255)            tGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGc                                                                <  1:147871/81‑1 (MQ=255)              gATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGt                                                              <  2:147872/81‑1 (MQ=255)                          gCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGtt                                                   >  2:147908/1‑80 (MQ=255)                           ccGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTg                                                  >  2:147916/1‑80 (MQ=255)                            cGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGa                                                 >  1:147934/1‑80 (MQ=255)                                  cAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGt                                            >  1:147928/1‑79 (MQ=255)                                   aGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAg                                         >  2:147924/1‑81 (MQ=255)                                    gCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAgg                                        <  2:147873/81‑1 (MQ=255)                                        taatCTGCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCt                                    >  1:147917/1‑81 (MQ=255)                                              gCCACCACTGCGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAata                              >  2:147933/1‑81 (MQ=255)                                                         gTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGt                   >  2:147926/1‑81 (MQ=255)                                                         gTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTGAGGGt                   <  2:147874/81‑1 (MQ=255)                                                            ttATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTg                 <  1:147875/80‑1 (MQ=255)                                                              aTGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAg              >  1:147922/1‑81 (MQ=255)                                                                     aTCTCGGGGTAATGGCGGCGGAGCGTGCTGGAGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTac        <  2:147876/80‑1 (MQ=255)                                                                        tcGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTacaaca    >  2:147929/1‑81 (MQ=255)                                                                          ggggTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACAtt  >  1:147940/1‑81 (MQ=255)                                                                          ggggTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACAtt  >  1:147939/1‑81 (MQ=255)                                                                          ggggTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACAtt  >  1:147932/1‑81 (MQ=255)                                                                          ggggTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACAtt  <  1:147877/81‑1 (MQ=255)                                                                             |                                                                              GCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATT  >  NC_000913/696135‑696288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑