Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	104,426	C→T	L149F (CTT→TTT)	ftsA →	cell division protein FtsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	104,426	0	C	T	100.0%	155.7 / NA	42	L149F (CTT→TTT)	ftsA	cell division protein FtsA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/25);  total (17/25)

GATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGCG  >  NC_000913/104348‑104503                                                                               |                                                                              gATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAttt                                                                             <  2:24493/81‑1 (MQ=255) gATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAttt                                                                             >  1:24572/1‑81 (MQ=255)  aTGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAtttt                                                                            >  2:24557/1‑81 (MQ=255)   tGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTc                                                                           <  1:24494/81‑1 (MQ=255)       cATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGc                                                                       >  1:24552/1‑81 (MQ=255)         tCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGt                                                                     <  1:24495/81‑1 (MQ=255)           gtgtGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGc                                                                   <  1:24496/81‑1 (MQ=255)              tgctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCgg                                                                 <  2:24497/80‑1 (MQ=255)                ctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGAt                                                               <  2:24500/80‑1 (MQ=255)                ctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGAt                                                               <  1:24498/80‑1 (MQ=255)                ctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGAt                                                               <  1:24499/80‑1 (MQ=255)                ctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATg                                                              >  1:24569/1‑81 (MQ=255)                        gATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCaa                                                       >  2:24560/1‑80 (MQ=255)                         aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCaaa                                                      <  2:24501/80‑1 (MQ=255)                             cGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGc                                                 <  2:24502/81‑1 (MQ=255)                              gCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCa                                                >  1:24576/1‑81 (MQ=255)                                    gTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGAt                                          <  1:24503/81‑1 (MQ=255)                                       tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcac                                       <  1:24504/81‑1 (MQ=255)                                        gCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcaca                                      >  1:24549/1‑81 (MQ=255)                                            ttGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTc                                  <  2:24505/81‑1 (MQ=255)                                              gACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTca                                 <  1:24506/80‑1 (MQ=255)                                                  aTCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAAc                             >  1:24559/1‑80 (MQ=255)                                                  aTCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAAc                             >  2:24571/1‑80 (MQ=255)                                                    cAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa                           >  2:24565/1‑80 (MQ=255)                                                     aGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGata                         <  2:24508/81‑1 (MQ=255)                                                     aGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGata                         <  1:24507/81‑1 (MQ=255)                                                     aGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGat                          <  1:24509/80‑1 (MQ=255)                                                      ggAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGatat                        >  1:24568/1‑81 (MQ=255)                                                        aaGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATgg                      >  1:24593/1‑81 (MQ=255)                                                         aGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGc                     <  2:24510/81‑1 (MQ=255)                                                           ggATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGa                   <  2:24511/81‑1 (MQ=255)                                                                aaGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa               >  2:24553/1‑80 (MQ=255)                                                                aaGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa               >  2:24583/1‑80 (MQ=255)                                                                aaGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa               >  1:24566/1‑80 (MQ=255)                                                                 aGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACa             >  1:24558/1‑81 (MQ=255)                                                                  gAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACAt            <  2:24512/81‑1 (MQ=255)                                                                   aaTCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATc           >  2:24564/1‑81 (MQ=255)                                                                    aTCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCg          >  1:24574/1‑81 (MQ=255)                                                                     tCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGt         <  1:24513/81‑1 (MQ=255)                                                                      ccGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGt         >  1:24582/1‑80 (MQ=255)                                                                       cGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTc        <  1:24514/80‑1 (MQ=255)                                                                       cGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCa       <  1:24515/81‑1 (MQ=255)                                                                         gTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCaaa     <  1:24516/81‑1 (MQ=255)                                                                            ggATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGc   <  2:24517/80‑1 (MQ=255)                                                                            ggATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGCg  <  1:24518/81‑1 (MQ=255)                                                                               |                                                                              GATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGCG  >  NC_000913/104348‑104503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑