Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	698,541	G→T	R213S (CGT→AGT)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	698,541	0	G	T	100.0%	128.5 / NA	36	R213S (CGT→AGT)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/21);  total (15/21)

GACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGC  >  NC_000913/698466‑698621                                                                            |                                                                                 gACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCt                                                                             <  2:148334/81‑1 (MQ=255)         gAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTg                                                                     <  1:148335/81‑1 (MQ=255)                      cGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTAt                                                        <  1:148336/81‑1 (MQ=255)                       gTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATc                                                       <  1:148337/81‑1 (MQ=255)                         aaGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCtt                                                     >  2:148379/1‑81 (MQ=255)                           ggCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCttt                                                    >  1:148397/1‑80 (MQ=255)                              acatcagacatcagATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCAcc                                                <  1:148338/81‑1 (MQ=255)                                   agacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCAt                                            <  1:148339/80‑1 (MQ=255)                                     acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCg                                          >  1:148377/1‑80 (MQ=255)                                     acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCg                                          >  1:148386/1‑80 (MQ=255)                                     acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGt                                         <  2:148340/81‑1 (MQ=255)                                        tcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGt                                      >  2:148376/1‑81 (MQ=255)                                          agaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTc                                     <  2:148341/80‑1 (MQ=255)                                               gCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAAcc                                <  1:148344/80‑1 (MQ=255)                                               gCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAAcc                                <  2:148343/80‑1 (MQ=255)                                               gCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAAcc                                <  2:148345/80‑1 (MQ=255)                                               gCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCa                               <  2:148342/81‑1 (MQ=255)                                               gCTTTTAACTGAATCATCCAGTGCCGGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAAcc                                <  1:148346/80‑1 (MQ=255)                                                   ttAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTc                            <  2:148347/80‑1 (MQ=255)                                                   ttAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTc                            <  2:148348/80‑1 (MQ=255)                                                     aaCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcgc                          <  2:148349/80‑1 (MQ=255)                                                     aaCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcgc                          >  2:148387/1‑80 (MQ=255)                                                      aCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcgcg                         <  2:148350/80‑1 (MQ=255)                                                      aCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCga                        >  2:148381/1‑81 (MQ=255)                                                       cTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgat                       <  2:148351/81‑1 (MQ=255)                                                         gAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatga                     >  1:148396/1‑81 (MQ=255)                                                         gAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCcatga                     >  1:148384/1‑81 (MQ=255)                                                          aaTCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatga                     >  1:148382/1‑80 (MQ=255)                                                          aaTCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatga                     >  1:148398/1‑80 (MQ=255)                                                               tCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGt                 >  2:148392/1‑79 (MQ=255)                                                                ccAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTaa               <  2:148352/80‑1 (MQ=255)                                                                ccAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTaa               <  1:148353/80‑1 (MQ=255)                                                                  aGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGaa            >  2:148389/1‑81 (MQ=255)                                                                   gTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAAc           >  1:148401/1‑81 (MQ=255)                                                                       cTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAt       <  2:148354/81‑1 (MQ=255)                                                                         gACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAttt     >  2:148391/1‑81 (MQ=255)                                                                            gCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGc  <  1:148356/81‑1 (MQ=255)                                                                            |                                                                                 GACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGC  >  NC_000913/698466‑698621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑