Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	724,054	G→A	H121Y (CAT→TAT)	kdpD ←	sensory histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	724,054	0	G	A	100.0%	137.5 / NA	38	H121Y (CAT→TAT)	kdpD	sensory histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/22);  total (16/22)

TGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCG  >  NC_000913/723978‑724130                                                                             |                                                                             tGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACgg                                                                          >  2:153222/1‑81 (MQ=255)  gCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACgg                                                                          >  2:153247/1‑80 (MQ=255)  gCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACgg                                                                          >  2:153251/1‑80 (MQ=255)     gAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAAc                                                                      >  2:153253/1‑81 (MQ=255)        cGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTg                                                                   >  1:153241/1‑81 (MQ=255)         gTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTgg                                                                  >  1:153238/1‑81 (MQ=255)          ttGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGgc                                                                 <  1:153187/81‑1 (MQ=255)            gACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGgcg                                                                <  1:153188/80‑1 (MQ=255)               ggTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCAtt                                                            <  2:153189/81‑1 (MQ=255)                  aGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACt                                                         >  1:153249/1‑81 (MQ=255)                     gAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACtgtg                                                      >  2:153248/1‑81 (MQ=255)                      aaaaCATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTgc                                                     <  1:153191/81‑1 (MQ=255)                      aaaaCATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTgc                                                     <  2:153190/81‑1 (MQ=255)                       aaaCATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTgcg                                                    >  1:153245/1‑81 (MQ=255)                         aCATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGcc                                                  <  2:153192/81‑1 (MQ=255)                          cATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCa                                                 <  1:153193/81‑1 (MQ=255)                              aaTGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGtt                                              <  1:153194/80‑1 (MQ=255)                                     gCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCAt                                       >  1:153254/1‑80 (MQ=255)                                      cTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCAtt                                      >  1:153244/1‑80 (MQ=255)                                             cagTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAg                              >  2:153259/1‑81 (MQ=255)                                             cagTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAg                              <  1:153195/81‑1 (MQ=255)                                              agTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAgc                             <  1:153196/81‑1 (MQ=255)                                               gTTCTTCGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAgcg                            <  1:153197/81‑1 (MQ=255)                                                ttcttcGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAgcgc                           >  1:153252/1‑81 (MQ=255)                                                ttcttcGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAgcgc                           <  1:153198/81‑1 (MQ=255)                                                  cttcGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGcc                          <  2:153199/80‑1 (MQ=255)                                                   ttcGATATCCTGCCAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCgg                        >  2:153257/1‑81 (MQ=255)                                                               ccAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCggcgggcg              <  1:153200/79‑1 (MQ=255)                                                               ccAGCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCggcgggcg              <  2:153201/79‑1 (MQ=255)                                                                  gCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGggc         <  1:153202/81‑1 (MQ=255)                                                                  gCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGggc         <  1:153203/81‑1 (MQ=255)                                                                  gCGTTTGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGggc         >  1:153250/1‑81 (MQ=255)                                                                     tttGGGATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGggcggc      <  1:153204/81‑1 (MQ=255)                                                                        gggATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCAt    <  1:153205/80‑1 (MQ=255)                                                                        gggATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATc   >  1:153270/1‑81 (MQ=255)                                                                         ggATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATc   <  1:153206/80‑1 (MQ=255)                                                                         ggATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATc   <  1:153207/80‑1 (MQ=255)                                                                         ggATAACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCg  <  1:153208/81‑1 (MQ=255)                                                                             |                                                                             TGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCG  >  NC_000913/723978‑724130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑