| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 737,651 | A→G | pseudogene (752/1062 nt) | ybfL → | putative transposase YbfL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 737,651 | 0 | A | G | 93.3% | 38.2 / ‑3.6 | 15 | pseudogene (752/1062 nt) | ybfL | putative transposase YbfL |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (6/8); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
ACGTTTGAATGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAAC > NC_000913/737575‑737727 | aCGTTTGAATGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGc > 1:155836/1‑79 (MQ=255) gTTTGAATGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAg > 2:155823/1‑80 (MQ=255) gTTTGAATGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGa < 2:155804/81‑1 (MQ=255) tGAATGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGaaa < 1:155805/80‑1 (MQ=255) gAATGGAAAGGACTGAAGAAATTATGCGTGACAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGAAAt < 2:155806/80‑1 (MQ=255) aaTGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGAAATGa < 2:155807/81‑1 (MQ=255) aaaGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGAAATGACGGTc < 2:155808/81‑1 (MQ=255) tATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGAAATGACGGTCAGATATTATATCAGtt > 1:155827/1‑81 (MQ=255) tGCGTGGCAGTCTCC‑TTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGAAATGACGGTCAGATATTATATCAGTTctg < 1:155809/81‑1 (MQ=255) ggCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGa > 1:155842/1‑81 (MQ=18) aGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGAtt > 1:155832/1‑80 (MQ=21) gTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTa < 1:155810/81‑1 (MQ=18) tCGGTCAATAATAGCAGAACAAAAGAAAGGGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAg > 2:155835/1‑80 (MQ=255) ataataGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTc < 2:155811/81‑1 (MQ=255) cAAAAGAAAGGGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATc < 2:155812/81‑1 (MQ=255) aagaaaggGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGa < 2:155813/81‑1 (MQ=255) agaaaggGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGa > 1:155850/1‑80 (MQ=255) aaggGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAAc > 1:155839/1‑80 (MQ=255) | ACGTTTGAATGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAAC > NC_000913/737575‑737727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |