Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	738,053	A→C	intergenic (+92/‑39)	ybfL → / → ybfD	putative transposase YbfL/H repeat‑associated putative transposase YbfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	738,053	0	A	C	100.0%	32.3 / NA	11	intergenic (+92/‑39)	ybfL/ybfD	putative transposase YbfL/H repeat‑associated putative transposase YbfD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/5);  total (6/5)

TTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTAAAAAATTGATGGAACATATTTC  >  NC_000913/737989‑738123                                                                 |                                                                       ttCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATCCATGATCAAATActtc                                                        <  1:155864/81‑1 (MQ=17)  tCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATCCATGATCAAATActtct                                                       >  2:155882/1‑81 (MQ=18)                      ttGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATCCATGATCAAATACTTCTTACATAATTAAGGAGAACaa                                   <  2:155865/81‑1 (MQ=255)                            tatTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATCCATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGa                             <  2:155866/81‑1 (MQ=255)                                    attaCCTGAAGCTGTTTTTTATTGCTTATCCATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTaaa                      >  1:155878/1‑80 (MQ=255)                                    attaCCTGAAGCTGTTTTTTATTGCTTATCCATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTaaa                      >  1:155880/1‑80 (MQ=255)                                        ccTGAAGCTGTTTTTTATTGCTTATCCATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTAAAAAAtt                 <  2:155867/81‑1 (MQ=255)                                        ccTGAAGCTGTTTTTTATTGCTTATCCATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTAAAAAAt                  <  2:155868/80‑1 (MQ=255)                                                 gTTTTTTATTGCTTATCCATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTAAAAAATTGATGGAACa        >  1:155884/1‑81 (MQ=255)                                                     tttATTGCTTATCCATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTAAAAAATTGATGGAACATAtt    >  1:155886/1‑81 (MQ=255)                                                        aTTGCTTATCCATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTAAAAAATTGATGGAACATATTTc  >  2:155879/1‑80 (MQ=255)                                                                 |                                                                       TTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTAAAAAATTGATGGAACATATTTC  >  NC_000913/737989‑738123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑