Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	792,039	G→A	T6I (ACC→ATC)	galE ←	UDP‑glucose 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	792,039	0	G	A	100.0%	111.5 / NA	31	T6I (ACC→ATC)	galE	UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/19);  total (12/19)

TCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAG  >  NC_000913/791964‑792114                                                                            |                                                                            tCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAAcc                                                                        <  1:166352/81‑1 (MQ=255)   aaGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAg                                                                      <  1:166353/81‑1 (MQ=255)    aGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGa                                                                     <  2:166354/81‑1 (MQ=255)     gAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGaa                                                                    >  1:166394/1‑81 (MQ=255)      aaTGATGACATCATGACCGTTTTGCAGTAATTGCACACTGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAAc                                                                   <  2:166355/81‑1 (MQ=255)      aaTGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAAc                                                                   >  2:166393/1‑81 (MQ=255)       atgatgACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAAct                                                                  <  2:166356/81‑1 (MQ=255)         gatgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAActct                                                                >  2:166400/1‑81 (MQ=255)          atgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACtctc                                                               <  2:166357/81‑1 (MQ=255)            gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCa                                                              >  1:166407/1‑80 (MQ=255)             aCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCAt                                                             <  1:166358/80‑1 (MQ=255)               atcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAAt                                                          <  1:166359/81‑1 (MQ=255)                     aCCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTc                                                    >  2:166405/1‑81 (MQ=255)                      ccGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTcc                                                   <  2:166360/81‑1 (MQ=255)                       cGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCACTCCa                                                  <  1:166361/81‑1 (MQ=255)                             gCAGTAATTCCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAgg                                             <  2:166362/80‑1 (MQ=255)                              cAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCt                                           <  1:166363/81‑1 (MQ=255)                               aGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCt                                           >  2:166403/1‑80 (MQ=255)                                gTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTa                                         >  2:166387/1‑81 (MQ=255)                                 tAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTAt                                        <  2:166364/81‑1 (MQ=255)                                    ttGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGt                                     <  1:166365/81‑1 (MQ=255)                                      gCACACAGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTAt                                   >  1:166404/1‑81 (MQ=255)                                       cacacaGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATg                                  <  2:166366/81‑1 (MQ=255)                                       cacacaGGTATGACTTCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATg                                  <  1:166367/81‑1 (MQ=255)                                                     ttCCAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCa                    >  1:166415/1‑81 (MQ=255)                                                       ccAATGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCAt                   >  2:166397/1‑80 (MQ=255)                                                           tGTAACCGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACaa              <  1:166368/81‑1 (MQ=255)                                                                 cGCTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGc        <  2:166369/81‑1 (MQ=255)                                                                   cTACCACCGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGa      <  1:166370/81‑1 (MQ=255)                                                                     accaccGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGaa     >  2:166417/1‑80 (MQ=255)                                                                       caccGATAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAg  >  2:166401/1‑81 (MQ=255)                                                                            |                                                                            TCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAG  >  NC_000913/791964‑792114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑