Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	797,736	T→C	E232E (GAA→GAG)	ybhA ←	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	797,736	0	T	C	100.0%	99.1 / NA	28	E232E (GAA→GAG)	ybhA	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/12);  total (16/12)

GTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCA  >  NC_000913/797658‑797813                                                                               |                                                                              gtTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTc                                                                             >  2:167542/1‑81 (MQ=255)     tCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAg                                                                          >  1:167549/1‑80 (MQ=255)     tCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGc                                                                         <  1:167503/81‑1 (MQ=255)            tCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGa                                                                  <  2:167504/81‑1 (MQ=255)              acaATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGat                                                                 <  1:167505/80‑1 (MQ=255)                 aTGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCa                                                             >  2:167540/1‑81 (MQ=255)                      ggCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTaa                                                         <  2:167506/80‑1 (MQ=255)                      ggCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTa                                                          >  2:167544/1‑79 (MQ=255)                           gcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTAt                                                   >  2:167556/1‑81 (MQ=255)                           gcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTAt                                                   <  2:167507/81‑1 (MQ=255)                                 ttACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCg                                              <  1:167508/80‑1 (MQ=255)                                  tACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGaa                                            >  2:167553/1‑81 (MQ=255)                                   aCCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAAt                                           >  1:167563/1‑81 (MQ=255)                                   aCCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAAt                                           >  2:167550/1‑81 (MQ=255)                                    ccGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAAt                                           <  2:167509/80‑1 (MQ=255)                                     cgcgTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGc                                         <  2:167510/81‑1 (MQ=255)                                         tCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCacg                                     <  2:167511/81‑1 (MQ=255)                                              ggCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGt                                 <  1:167512/80‑1 (MQ=255)                                                  ttGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTc                             >  1:167554/1‑80 (MQ=255)                                                        aTCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGAc                      <  1:167513/81‑1 (MQ=255)                                                        aTCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGAc                      >  2:167546/1‑81 (MQ=255)                                                         tCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGAc                      >  1:167543/1‑80 (MQ=255)                                                           gCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCaa                   >  1:167571/1‑81 (MQ=255)                                                           gCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCaa                   >  1:167560/1‑81 (MQ=255)                                                                 ccTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTgc             <  2:167514/81‑1 (MQ=255)                                                                  cTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTgcg            >  1:167548/1‑81 (MQ=255)                                                                          gCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAAcc    >  2:167567/1‑81 (MQ=255)                                                                            ggCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCa  >  1:167566/1‑81 (MQ=255)                                                                               |                                                                              GTTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCA  >  NC_000913/797658‑797813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑