| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 106,741 | T→G | L62R (CTC→CGC) | lpxC → | UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 106,741 | 0 | T | G | 96.9% | 109.6 / ‑4.0 | 32 | L62R (CTC→CGC) | lpxC | UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (17/14); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
GGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTG > NC_000913/106663‑106817 | gggTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGcgct < 1:25091/81‑1 (MQ=255) gTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTg > 1:25150/1‑80 (MQ=255) gTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTg > 1:25129/1‑80 (MQ=255) cATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTAc > 2:25161/1‑81 (MQ=255) tcgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACgt < 2:25092/77‑1 (MQ=255) cgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTg > 2:25144/1‑81 (MQ=255) gCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTc > 2:25151/1‑80 (MQ=255) cACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCaa > 2:25153/1‑81 (MQ=255) aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAAc > 2:25142/1‑81 (MQ=255) aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAAc < 2:25094/81‑1 (MQ=255) aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAAc < 2:25093/81‑1 (MQ=255) ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAAc > 1:25154/1‑80 (MQ=255) tCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCatgatg > 1:25141/1‑80 (MQ=255) tCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGt < 1:25095/81‑1 (MQ=255) cACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTAc < 2:25096/81‑1 (MQ=255) ccGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACgg < 2:25097/81‑1 (MQ=255) gTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAtt < 2:25098/81‑1 (MQ=255) aGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAttt > 1:25147/1‑80 (MQ=255) gATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTc < 1:25099/80‑1 (MQ=255) cccGGCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg < 1:25100/80‑1 (MQ=255) ggCCGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTa > 1:25145/1‑79 (MQ=255) cGATGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCa > 2:25163/1‑81 (MQ=255) aTGCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCAcc < 1:25101/81‑1 (MQ=255) gCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCt > 1:25139/1‑80 (MQ=255) gCCAAATCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCt > 2:25152/1‑80 (MQ=255) aaaTCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAAt < 2:25102/81‑1 (MQ=255) aaaTCTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAAt > 1:25162/1‑81 (MQ=255) cTGTGCGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAAtgctg > 1:25143/1‑81 (MQ=255) cGTGATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCtctc < 1:25103/81‑1 (MQ=255) gATACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTcgcg > 2:25155/1‑81 (MQ=255) aTACCATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTcgcg < 2:25104/80‑1 (MQ=255) cATGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCtt < 2:25105/81‑1 (MQ=255) aTGCGCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTg > 2:25158/1‑81 (MQ=255) | GGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTG > NC_000913/106663‑106817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |