Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	836,709	A→C	H120P (CAT→CCT)	hcxB →	hydroxycarboxylate dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	836,709	0	A	C	100.0%	88.4 / NA	25	H120P (CAT→CCT)	hcxB	hydroxycarboxylate dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/17);  total (8/17)

GCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGT  >  NC_000913/836639‑836781                                                                       |                                                                         gCGATGGCGCTGGGGATTGAGAAAGCGCATAGGCACGGTATTGCCGCCGTGGAGCTACATAACTCGCATCCTATCGGCCGt                                                                <  2:175202/81‑1 (MQ=255)     tGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCg                                                            <  2:175203/81‑1 (MQ=255)      ggCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCgg                                                           >  1:175242/1‑81 (MQ=255)            ggggATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTg                                                     >  1:175243/1‑81 (MQ=255)               gATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGc                                                  <  2:175204/81‑1 (MQ=255)                 ttGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCgg                                                <  1:175205/81‑1 (MQ=255)                    agaAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGc                                             <  2:175207/81‑1 (MQ=255)                    agaAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGc                                             <  2:175206/81‑1 (MQ=255)                    agaAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGc                                             <  1:175208/81‑1 (MQ=255)                     gaAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAg                                              >  1:322782/1‑79 (MQ=255)                       aaGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAgt                                          >  2:175246/1‑81 (MQ=255)                       aaGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAgt                                          <  2:175209/81‑1 (MQ=255)                       aaGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAgt                                          <  1:175210/81‑1 (MQ=255)                           gcATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGc                                      <  1:175211/81‑1 (MQ=255)                                aGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAgcgg                                 <  2:175212/81‑1 (MQ=255)                                 gCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAgcggc                                >  2:175250/1‑81 (MQ=255)                                 gCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAgcggc                                <  1:175213/81‑1 (MQ=255)                                  cACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAgcggcg                               <  1:175214/81‑1 (MQ=255)                                   aCGGTATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGcggcgg                              >  1:175252/1‑81 (MQ=255)                                       tATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGtt                          >  1:175251/1‑81 (MQ=255)                                       tATTGCCGCCGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGtt                          <  1:175215/81‑1 (MQ=255)                                           gccgccGTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGtc                      >  2:175257/1‑81 (MQ=255)                                              gccgTGGCGCTACATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTctct                   <  2:175216/81‑1 (MQ=255)                                                          cATAACTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTg         <  1:175217/79‑1 (MQ=255)                                                               cTCGCATCCTATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGt  <  1:175218/81‑1 (MQ=255)                                                                       |                                                                         GCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGT  >  NC_000913/836639‑836781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑