Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	844,647	G→A	G278G (GGC→GGT)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	844,647	0	G	A	100.0%	82.9 / NA	24	G278G (GGC→GGT)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/13);  total (11/13)

ATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAAC  >  NC_000913/844573‑844722                                                                           |                                                                            aTAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATaa                                                                       <  1:176571/81‑1 (MQ=255)   aaTGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAAcc                                                                     >  1:176617/1‑81 (MQ=255)       atgaCGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTAt                                                                  <  1:176572/80‑1 (MQ=255)        tgaCGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCa                                                                <  1:176573/81‑1 (MQ=255)          aCGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCaaa                                                              >  2:176618/1‑81 (MQ=255)                      gcACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAg                                                  <  2:176574/81‑1 (MQ=255)                       cACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGc                                                 >  1:176613/1‑81 (MQ=255)                       cACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGc                                                 >  2:176611/1‑81 (MQ=255)                         ccTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCt                                                >  2:176619/1‑80 (MQ=255)                         ccTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTc                                               <  1:176575/81‑1 (MQ=255)                          cTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCt                                                >  2:176622/1‑79 (MQ=255)                                 tACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGcgacg                                       <  1:176576/81‑1 (MQ=255)                                   cATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCgacgac                                     <  1:176577/81‑1 (MQ=255)                                        aCCTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAg                                >  1:176639/1‑81 (MQ=255)                                          cTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGt                               >  2:176623/1‑80 (MQ=255)                                          cTGATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTa                              <  2:176578/81‑1 (MQ=255)                                            gATTAGAGATAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAAc                            >  2:176620/1‑81 (MQ=255)                                                 gagaTAATCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGgc                        <  2:176579/80‑1 (MQ=255)                                                       aTCGGCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCtctc                  <  2:176580/80‑1 (MQ=255)                                                          ggCACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTcc              >  1:176624/1‑81 (MQ=255)                                                            cACGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTg            <  1:176581/81‑1 (MQ=255)                                                             aCGGCCACAATCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGa           <  2:176582/81‑1 (MQ=255)                                                                     aaTCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGaa   <  2:176583/81‑1 (MQ=255)                                                                      aTCAGACCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAAc  >  2:176634/1‑81 (MQ=255)                                                                           |                                                                            ATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAAC  >  NC_000913/844573‑844722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑