Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	881,801	A→G	N359D (AAC→GAC)	dacC →	D‑alanyl‑D‑alanine carboxypeptidase DacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	881,801	0	A	G	100.0%	96.3 / NA	27	N359D (AAC→GAC)	dacC	D‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

ATACGTTAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTAACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGCGGATTCTTTGG  >  NC_000913/881727‑881868                                                                           |                                                                    ataCGTTAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGt                                                                >  1:183347/1‑80 (MQ=255)   aCGTTAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTaaa                                                             >  2:183351/1‑81 (MQ=255)     gTTAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATc                                                           >  2:183339/1‑81 (MQ=255)      ttAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATcc                                                          <  1:183302/81‑1 (MQ=255)          cGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTg                                                      <  2:183303/81‑1 (MQ=255)           agAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCAtt                                                       >  1:183358/2‑79 (MQ=255)                cGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGagcagc                                                >  2:183349/1‑81 (MQ=255)                    gCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTcc                                            <  1:183304/81‑1 (MQ=255)                     cTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCg                                           >  2:183341/1‑81 (MQ=255)                     cTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCg                                           <  1:183305/81‑1 (MQ=255)                           gcaccgcTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATc                                     >  2:183356/1‑81 (MQ=255)                             accgcTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGt                                   <  2:183306/81‑1 (MQ=255)                              ccgcTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTg                                  <  1:183307/81‑1 (MQ=255)                                 cTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATg                               >  1:183357/1‑81 (MQ=255)                                     aaaaaGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGaaa                           <  2:183308/81‑1 (MQ=255)                                     aaaaaGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGaa                            >  1:183352/1‑80 (MQ=255)                                              aGGTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAag                  <  1:183309/81‑1 (MQ=255)                                                gTTGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAagag                <  2:183310/81‑1 (MQ=255)                                                 ttGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAgg               <  2:183311/81‑1 (MQ=255)                                                  tGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAggg              >  1:183361/1‑81 (MQ=255)                                                  tGTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAggg              >  1:183344/1‑81 (MQ=255)                                                   gTCGGGACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGc             >  2:183362/1‑81 (MQ=255)                                                       ggACCATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGCGGAt         >  1:183350/1‑81 (MQ=255)                                                           cATTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGCGGATTCtt     >  1:183354/1‑81 (MQ=255)                                                            aTTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGCGGATTCttt    >  2:183346/1‑81 (MQ=255)                                                            aTTGATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGCGGATTCttt    <  1:183312/81‑1 (MQ=255)                                                               gATTTCCAGCTTGACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGCGGATTCTTTgg  <  2:183313/80‑1 (MQ=255)                                                                           |                                                                    ATACGTTAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTAACGGTAAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGCGGATTCTTTGG  >  NC_000913/881727‑881868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑