| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 897,586 | G→A | R168H (CGC→CAC) | potI → | putrescine ABC transporter membrane subunit PotI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 897,586 | 0 | G | A | 97.3% | 126.6 / ‑3.7 | 37 | R168H (CGC→CAC) | potI | putrescine ABC transporter membrane subunit PotI |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/24); minor base T (0/1); total (12/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
ATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTACCGATGATCA > NC_000913/897510‑897663 | aTCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCg < 2:186058/81‑1 (MQ=255) ggctggcGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGa > 2:186108/1‑78 (MQ=255) gctggcGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGa < 2:186061/77‑1 (MQ=255) gctggcGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATaga < 2:186060/81‑1 (MQ=255) gctggcGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATa < 1:186059/79‑1 (MQ=255) cgcATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGaagaag < 1:186062/81‑1 (MQ=255) gcATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAagc < 2:186064/81‑1 (MQ=255) gcATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAagc < 2:186063/81‑1 (MQ=255) cATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAagca < 2:186065/81‑1 (MQ=255) aTGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAagcag < 1:186066/81‑1 (MQ=255) gTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAagcagc > 2:186114/1‑80 (MQ=255) aCTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATg > 2:186113/1‑81 (MQ=255) cTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCGAGATTGCGGAAACTGGATCACTCGATAGAAGAAACAGCGATgg < 2:186067/81‑1 (MQ=255) tttGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGa < 2:186068/79‑1 (MQ=255) ttGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGAtct > 2:186111/1‑81 (MQ=255) ggCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGc < 1:186070/81‑1 (MQ=255) ggCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGc < 2:186069/81‑1 (MQ=255) aTGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGAc < 1:186071/79‑1 (MQ=255) aTGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCGCGGTGCGAcg < 2:186072/80‑1 (MQ=255) ggCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCt > 2:186122/1‑81 (MQ=255) gCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCt > 2:186131/1‑80 (MQ=255) gCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTg < 1:186073/81‑1 (MQ=255) ttATTTCTTCAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAgtgt < 1:186074/81‑1 (MQ=255) ttcttcAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGttttt > 1:186116/1‑81 (MQ=255) cttcAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCg > 1:186123/1‑81 (MQ=255) cAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGt < 2:186076/79‑1 (MQ=255) cAAGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTc < 2:186075/80‑1 (MQ=255) aaGATTGCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCa < 1:186077/80‑1 (MQ=255) gCGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGc > 1:186115/1‑80 (MQ=255) cGGGAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTa > 2:186125/1‑81 (MQ=255) gggAACTGGATCTCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTAc < 2:186078/81‑1 (MQ=255) gggAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTAc > 2:186118/1‑81 (MQ=255) gggAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTAc > 1:186117/1‑81 (MQ=255) ggAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTTATTACGCTAcc < 1:186079/81‑1 (MQ=255) ggAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTAcc < 2:186080/81‑1 (MQ=255) gAACTGGATCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTACCg < 2:186081/81‑1 (MQ=255) aTCACTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTACCGATGATCa < 2:186082/81‑1 (MQ=255) | ATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTACCGATGATCA > NC_000913/897510‑897663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |