Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	914,159	T→C	E233E (GAA→GAG)	lysO ←	L‑lysine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	914,159	0	T	C	100.0%	92.9 / NA	26	E233E (GAA→GAG)	lysO	L‑lysine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/18);  total (8/18)

GATGTGGCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATAAAATACC  >  NC_000913/914082‑914230                                                                              |                                                                        gATGTGGCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTc                                                                       <  1:189242/80‑1 (MQ=255) gATGTGGCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTc                                                                       <  1:189241/80‑1 (MQ=255) gATGTGGCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTc                                                                       <  1:189243/80‑1 (MQ=255)  aTGTGGCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCa                                                                      <  1:189244/80‑1 (MQ=255)    gtgGCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCTAGGGATCAACATAATAGCAATCAGCTCACgg                                                                   >  2:189286/1‑81 (MQ=255)     tgGCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACgg                                                                   <  1:189245/80‑1 (MQ=255)                 aGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTa                                                       <  1:189246/80‑1 (MQ=255)                      aGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAAc                                                 >  1:189282/1‑81 (MQ=255)                        tGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAAcgc                                               <  2:189247/81‑1 (MQ=255)                          cAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAAcgccg                                             >  2:189280/1‑81 (MQ=255)                          cAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAAcgccg                                             <  2:189249/81‑1 (MQ=255)                          cAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAAcgccg                                             <  2:189248/81‑1 (MQ=255)                          cAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAAcgcc                                              <  1:189250/80‑1 (MQ=255)                            gTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCcgcg                                           <  2:189251/81‑1 (MQ=255)                                  cGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCg                                     <  1:189252/81‑1 (MQ=255)                                   ggcggcggcGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGa                                    <  2:189253/81‑1 (MQ=255)                                        cggcgAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTAcc                               >  2:189287/1‑81 (MQ=255)                                            gAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGAc                           >  1:189279/1‑81 (MQ=255)                                             aaTCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGAcc                          <  2:189254/81‑1 (MQ=255)                                                      aGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTc                 <  2:189255/81‑1 (MQ=255)                                                         gATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGt              >  1:189288/1‑81 (MQ=255)                                                          aTCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTc             <  1:189256/81‑1 (MQ=255)                                                             aaCATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCaa           >  1:189278/1‑80 (MQ=255)                                                                 taataGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATaaaa      <  1:189257/81‑1 (MQ=255)                                                                    taGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATAAAATAc   <  2:189258/81‑1 (MQ=255)                                                                     aGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATAAAATAcc  >  1:189284/1‑81 (MQ=255)                                                                              |                                                                        GATGTGGCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATAAAATACC  >  NC_000913/914082‑914230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑