| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,781,145 | 0 | C | G | 22.2% | 119.1 / 14.3 | 45 | intergenic (+128/‑70) | lldD/trmL | L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (24/11); new base G (0/10); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAT > NC_000913/3781065‑3781225 | cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGcc < 2:821325/81‑1 (MQ=255) gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑g > 2:821390/1‑81 (MQ=255) ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑gg < 2:821326/81‑1 (MQ=255) tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑aaa < 1:821328/81‑1 (MQ=255) tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑aaa < 2:821327/81‑1 (MQ=255) taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑aaaa < 1:821329/80‑1 (MQ=255) tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCAc > 1:821409/1‑81 (MQ=255) aCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTg > 2:821388/1‑81 (MQ=255) aCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTg > 1:821397/1‑81 (MQ=255) ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCt > 1:821405/1‑80 (MQ=255) gaaaaCAACCCCCCCCTTTTTTTTCCCCCCCCCCCCCTCGGGGGGGGGGGCG‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTc < 1:821338/78‑1 (MQ=255) gAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCAc > 2:821407/1‑81 (MQ=255) aaaCGACACCCCACTTTTTTTTTCCCCCCCCCCCCCCGGGGGAGGGGGCG‑GGGGTGGGGGG‑AAAAGGCCGCACTGCTc < 2:821330/78‑1 (MQ=255) cGACACCCACCTTTTTTTTCCCCCCCCCCCCCTCGGGGGAGGGGCCG‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTCACCAAt < 2:821337/81‑1 (MQ=255) acacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GTGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAAttt < 2:821331/81‑1 (MQ=255) acacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAAttt > 1:821391/1‑81 (MQ=255) acCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTc > 2:821392/1‑81 (MQ=255) aCTTTTTTTTTCCCCCCCCCCCCCCGGGGGAG‑GGGCC‑GGGGGGGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTa < 2:821332/81‑1 (MQ=255) aCTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTa > 2:821393/1‑81 (MQ=255) tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATc > 1:821410/1‑81 (MQ=255) ttttCTCCCTCGCCCCCCGGGGGGGGGGCCC‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGc < 2:821335/80‑1 (MQ=255) ttttCCCCCCCCCCCCCCCGGGGGGGGGGGC‑GGGGGGGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg < 1:821334/80‑1 (MQ=255) tccccccCGCCCCGCGGGGGGGGGGGAG‑GGGGGGGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc < 1:821355/77‑1 (MQ=255) tCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt > 2:821395/1‑80 (MQ=255) cGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt > 2:821401/1‑79 (MQ=255) cGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt > 2:821412/1‑79 (MQ=255) gCCCCTCGGGGGAGGGGGCG‑GGGGGGGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa < 2:821336/80‑1 (MQ=255) gCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg > 2:821399/1‑81 (MQ=255) gCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg > 2:821413/1‑81 (MQ=255) gCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg > 1:821402/1‑81 (MQ=255) cccccccCGGGGGGGGGGCCGGGGGTGGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa < 2:821340/80‑1 (MQ=255) ccccccGGGGGAGGGGGCG‑GGGGGGGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg < 1:821343/81‑1 (MQ=255) cccccGGGGGGGGGGCG‑GGGGGGGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg < 2:821342/78‑1 (MQ=255) ccTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg > 2:821403/1‑81 (MQ=255) cccggggaAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg < 2:821341/77‑1 (MQ=255) cTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg > 2:821411/1‑81 (MQ=255) tCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg > 1:821396/1‑80 (MQ=255) ccGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg > 1:821400/1‑79 (MQ=255) cGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg > 1:821406/1‑81 (MQ=255) gggAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa > 2:821421/1‑81 (MQ=255) gagaGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATg > 2:821394/1‑81 (MQ=255) gggggCG‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt < 2:821352/79‑1 (MQ=255) gagGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc > 2:821398/1‑80 (MQ=255) ggggCG‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa < 2:821346/80‑1 (MQ=255) agGCCC‑GGGGTGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa < 2:821345/81‑1 (MQ=255) gggCG‑GGGGTGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa < 2:821347/81‑1 (MQ=255) c‑GGGGTGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt < 2:821348/81‑1 (MQ=255) | CGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAT > NC_000913/3781065‑3781225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |