Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,782,393	(C)5→4	coding (334/4581 nt)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,782,389	0	C	.	100.0%	128.9 / NA	28	coding (338/4581 nt)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/13);  total (15/13)

AATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTC  >  NC_000913/2782313‑2782464                                                                             |                                                                            aaTTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCATTCTCGATTGTCGTACCTGTGGAAATAcccca                                                                         >  2:511949/1‑80 (MQ=255)    tGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccatca                                                                      >  2:511946/1‑77 (MQ=255)         ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAAt                                                                >  2:511950/1‑81 (MQ=255)          tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAAtt                                                               >  2:535785/1‑81 (MQ=255)           ccATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAAtt                                                               <  2:511918/80‑1 (MQ=255)               tGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTg                                                          >  2:511947/1‑81 (MQ=255)                gCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGt                                                         >  1:511954/1‑81 (MQ=255)                  tGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCt                                                       >  2:511955/1‑81 (MQ=255)                    aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCt                                                       <  1:511919/79‑1 (MQ=255)                      aCCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCa                                                   >  1:511920/1‑81 (MQ=255)                        ccccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCAcc                                                 <  2:511921/81‑1 (MQ=255)                           cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGtt                                              <  1:511922/81‑1 (MQ=255)                               ataAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAAt                                           <  1:511923/80‑1 (MQ=255)                               ataAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATg                                          >  2:511968/1‑81 (MQ=255)                                taAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATgg                                         >  1:511951/1‑81 (MQ=255)                                 aaaCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGt                                        >  2:511958/1‑81 (MQ=255)                                     gTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGta                                    <  1:511924/81‑1 (MQ=255)                                             tGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                            >  2:511961/1‑81 (MQ=255)                                             tGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                            >  1:511962/1‑81 (MQ=255)                                              gCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                            <  1:511925/80‑1 (MQ=255)                                                  ctctCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGcc                       <  2:511926/81‑1 (MQ=255)                                                       gATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCcaca                  <  1:511927/81‑1 (MQ=255)                                                           gTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaaaa               <  2:511928/80‑1 (MQ=255)                                                            tCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAAtt             <  1:511930/81‑1 (MQ=255)                                                            tCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAAt              <  2:511929/80‑1 (MQ=255)                                                             cGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTg            >  1:511957/1‑81 (MQ=255)                                                              gTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGt           >  1:511953/1‑81 (MQ=255)                                                                  cTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt        >  1:511960/1‑80 (MQ=255)                                                                  cTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt        >  1:511959/1‑80 (MQ=255)                                                                       gAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTc  <  2:511931/81‑1 (MQ=255)                                                                             |                                                                            AATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTC  >  NC_000913/2782313‑2782464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑