| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 448,092 | Δ1 bp | coding (569/615 nt) | cyoC ← | cytochrome bo3 ubiquinol oxidase subunit 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 448,090 | 0 | A | . | 96.9% | 138.8 / ‑2.9 | 32 | coding (571/615 nt) | cyoC | cytochrome bo3 ubiquinol oxidase subunit 3 |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (19/12); total (20/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGG > NC_000913/448011‑448166 | aTGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCaa > 1:95325/1‑81 (MQ=255) aCGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACcaca > 1:95332/1‑81 (MQ=255) gcgcCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATc > 1:95327/1‑81 (MQ=255) cgccgcTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATc > 2:95347/1‑80 (MQ=255) cgccgcTGTGATCGGTAGAATGACTCATTACATCG‑CCCCAACAGATAAACCACAGTGAACAACCAGAACCAACCcccac > 2:95339/1‑78 (MQ=255) gccgcTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATc > 2:95348/1‑79 (MQ=255) gccgcTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATc > 1:95330/1‑79 (MQ=255) gcTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAgg > 1:95340/1‑80 (MQ=255) tgtgATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAg > 2:95345/1‑81 (MQ=255) gtgATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGt > 1:95357/1‑81 (MQ=255) tgATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAACCAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTg < 2:95291/81‑1 (MQ=255) gATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGc > 2:95338/1‑81 (MQ=255) aTCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGc < 2:95292/80‑1 (MQ=255) tAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAAc < 1:95293/81‑1 (MQ=255) aTTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAgg < 1:95294/79‑1 (MQ=255) aTTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGc < 1:95295/80‑1 (MQ=255) gtccccATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACatgatg < 2:95296/78‑1 (MQ=255) gCCCCCATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGc > 2:95349/1‑81 (MQ=255) ccATCAGATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGt < 2:95297/81‑1 (MQ=255) gATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGTACAGGCTCAGGCACATGATGCGGGTACGGt > 2:95352/1‑80 (MQ=255) gATAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGtt < 1:95298/81‑1 (MQ=255) tAAACAACAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAg > 2:95356/1‑81 (MQ=255) aacaacAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTg > 2:95353/1‑81 (MQ=255) aacaacAGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTg > 1:95365/1‑81 (MQ=255) acaGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTgg > 2:95343/1‑81 (MQ=255) acaGTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTgg > 1:95351/1‑81 (MQ=255) gTGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGt > 1:95344/1‑79 (MQ=255) tGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAg < 1:95300/81‑1 (MQ=255) tGAACACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAg < 2:95299/81‑1 (MQ=255) aaCACACAGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGc < 2:95301/81‑1 (MQ=255) cacaGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCcgc > 2:95346/1‑80 (MQ=255) cacaGATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCcgc > 1:95360/1‑80 (MQ=255) gATCC‑AACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACgg < 2:95302/81‑1 (MQ=255) | ATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGG > NC_000913/448011‑448166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |