Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,199,503	(A)5→4	coding (809/894 nt)	lit →	e14 prophage; cell death peptidase Lit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,199,499	0	A	.	100.0%	46.0 / NA	11	coding (805/894 nt)	lit	e14 prophage; cell death peptidase Lit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/2);  total (9/2)

TTTCATGCTACCCTGTCGGAAATGAAGAGTTGATTGAAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGCAAAAATATCAATGTGAATCTAGATGGGGAGTCCTTTTCATCGATTTTAGGTGAT  >  NC_000913/1199422‑1199552                                                                              |                                                      tttCATGCTACCCTGTCGGAAATGAAGAGTTGATTGAAGCTCTATGTACTGTGATGCTTCAATATCTTTTCCATGGCaaaa                                                    >  2:235777/1‑81 (MQ=255) tttCATGCTACCCTGTCGGAAATGAAGAGTTGATTGAAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGCaaaa                                                    <  2:235764/81‑1 (MQ=255)   tCATGCTACCCTGTCGGAAATGAAGAGTTGATTGAAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGCaaaata                                                  >  1:235782/1‑79 (MQ=255)   tCATGCTACCCTGTCGGAAATGAAGAGTTGATTGAAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGCaaaat                                                   <  2:235765/80‑2 (MQ=255)        cTACCCTGTCGGAAATGAAGAGTTGATTGAAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAAt                                            >  1:235786/1‑81 (MQ=255)                     aaTGAAGAGTTGATTGAAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATg                               <  1:235766/81‑1 (MQ=255)                       tGAAGAGTTGATTGAAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATggg                             >  2:235780/1‑81 (MQ=255)                              ttgattgaAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATGGGGAGTCCt                      >  1:235789/1‑81 (MQ=255)                                   tgaAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATGGGGAGTCCTTTTCa                 >  2:235783/1‑81 (MQ=255)                                     aaGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATGGGGAGTCCTTTTCATc               <  2:235767/81‑1 (MQ=255)                                         tctATGTACAGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATGGGGAGTCCTTTTCATCTAtt           >  2:235791/1‑81 (MQ=255)                                                aCAGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATGGGGAGTCCTTTTCATCGATTTTAGGt     >  2:235784/1‑80 (MQ=255)                                                 cAGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATGGGGAGTCCTTTTCATCGATTTTAGGTGa   >  2:235797/1‑81 (MQ=255)                                                  aGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATGGGGAGTCCTTTTCATCGATTTTAGGTGAt  >  1:235790/1‑81 (MQ=255)                                                  aGTGATGCTTCAATATCTTTTCCATGGC‑AAAATATCAATGTGAATCTAGATGGGGAGTCCTTTTCATCGATTTTAGGTGAt  >  1:235793/1‑81 (MQ=255)                                                                              |                                                      TTTCATGCTACCCTGTCGGAAATGAAGAGTTGATTGAAGCTCTATGTACAGTGATGCTTCAATATCTTTTCCATGGCAAAAATATCAATGTGAATCTAGATGGGGAGTCCTTTTCATCGATTTTAGGTGAT  >  NC_000913/1199422‑1199552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑