Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,634,630	(A)6→7	coding (399/1371 nt)	xseA →	exodeoxyribonuclease VII subunit XseA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,634,624	1	.	A	100.0%	97.8 / NA	27	Y131* (TAC→TAA)	xseA	exodeoxyribonuclease VII subunit XseA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (11/16);  total (11/16)

CCGGTGAAGGGCTGCTGCAACAGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATAC‑AAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATT  >  NC_000913/2634545‑2634699                                                                                 |                                                                            ccggTGAAGGGCTGCTGCAACAGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATAC‑a                                                                            <  1:482250/81‑1 (MQ=255)  cggTGAAGGGCTGCTGCAACAGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATAC‑a                                                                            >  1:482288/1‑80 (MQ=255)     tGAAGGGCTGCTGCAACAGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATAC‑aaaa                                                                         >  2:482290/1‑80 (MQ=255)         gggCTGCTGCAACAGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATAC‑aaaaaaacc                                                                    <  1:482251/81‑4 (MQ=255)               ctgcAACAGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTtcc                                                               <  1:482253/81‑1 (MQ=255)               ctgcAACAGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTtc                                                                <  2:482252/80‑1 (MQ=255)               ctgcAACAGAAGTACGAACAGCTCAAAGCAAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACtt                                                                 <  2:858525/79‑1 (MQ=255)                tgcAACAGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTtccc                                                              >  2:482289/1‑81 (MQ=255)                      aGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCt                                                        >  2:482285/1‑81 (MQ=255)                        aaGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTgc                                                      <  1:482254/81‑1 (MQ=255)                              gAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTg                                                >  1:482287/1‑81 (MQ=255)                                  aGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGtt                                            <  2:482255/81‑1 (MQ=255)                                       aaaGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGgtgt                                       <  1:482256/81‑1 (MQ=255)                                          gCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGAt                                    <  2:482257/81‑1 (MQ=255)                                          gCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGAt                                    <  1:482258/81‑1 (MQ=255)                                           cGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATc                                   <  2:482259/81‑1 (MQ=255)                                             aaGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCAc                                 >  1:482304/1‑81 (MQ=255)                                             aaGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCAc                                 <  1:482260/81‑1 (MQ=255)                                               gTTGCAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCt                               >  2:482295/1‑81 (MQ=255)                                                   cAGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCaaa                           <  1:482261/81‑1 (MQ=255)                                                    aGGCTGAAGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCaaa                           >  2:482298/1‑80 (MQ=255)                                                          aaGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGt                    >  2:482303/1‑81 (MQ=255)                                                           aGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGtg                   >  1:482306/1‑81 (MQ=255)                                                           aGGTTTGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGtg                   <  2:482262/81‑1 (MQ=255)                                                                tGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGtgctgc               <  2:482263/80‑1 (MQ=255)                                                                tGTTCGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTgcg              >  2:482297/1‑81 (MQ=255)                                                                    cGATCAGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTa           >  1:482296/1‑80 (MQ=255)                                                                         aGCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGa      <  1:482264/80‑1 (MQ=255)                                                                          gCAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGata    >  2:482308/1‑81 (MQ=255)                                                                           cAATACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGatat   <  1:482265/81‑1 (MQ=255)                                                                            aaTACAAAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATAtt  <  1:482266/81‑1 (MQ=255)                                                                                 |                                                                            CCGGTGAAGGGCTGCTGCAACAGAAGTACGAACAGCTCAAAGCGAAGTTGCAGGCTGAAGGTTTGTTCGATCAGCAATAC‑AAAAAACCACTTCCCTCCCCTGCGCATTGCGTTGGTGTGATCACCTCAAAAACCGGTGCTGCGCTACATGATATT  >  NC_000913/2634545‑2634699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑