Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,432,299	+C	coding (166/474 nt)	smg ←	DUF494 domain‑containing protein Smg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,432,296	1	.	C	100.0%	65.8 / NA	19	L57V (TTG→GTG)	smg	DUF494 domain‑containing protein Smg
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/10);  total (9/10)

TTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑CCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGT  >  NC_000913/3432228‑3432370                                                                      |                                                                           ttCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGAt                                                                 <  1:653311/81‑1 (MQ=255) ttCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGAt                                                                 <  1:653310/81‑1 (MQ=255)       ctctTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAg                                                           >  1:653344/1‑81 (MQ=255)                    taAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCa                                               <  1:653312/80‑1 (MQ=255)                     aaaTACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGc                                             >  1:820890/1‑81 (MQ=255)                      aaTACGCATGGAGAGAGGATCTGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGc                                             >  2:653349/1‑80 (MQ=255)                      aaTACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGc                                             >  1:653345/1‑80 (MQ=255)                       aTACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCa                                           <  1:653313/81‑1 (MQ=255)                            cATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAg                                        >  1:653359/1‑79 (MQ=255)                             aTGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAg                                     <  2:653314/81‑1 (MQ=255)                              tGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAgg                                    >  2:653350/1‑81 (MQ=255)                                  gagagGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCAt                                >  1:653346/1‑81 (MQ=255)                                   agagGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCAtt                               <  2:653315/81‑1 (MQ=255)                                     agGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTg                              >  2:653351/1‑80 (MQ=255)                                           cGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGatat                       <  2:653316/81‑1 (MQ=255)                                           cGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGatat                       <  2:653317/81‑1 (MQ=255)                                               gCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATAtcttc                   <  1:653318/81‑1 (MQ=255)                                                             ttCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTg     <  1:653319/81‑1 (MQ=255)                                                                tGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGt  >  2:653358/1‑81 (MQ=255)                                                                      |                                                                           TTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑CCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGT  >  NC_000913/3432228‑3432370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑