| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 361,727 | (T)6→7 | coding (134/612 nt) | lacA ← | galactoside O‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 361,721 | 1 | . | T | 92.9% | 44.1 / ‑2.3 | 14 | coding (140/612 nt) | lacA | galactoside O‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (7/6); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTC‑TT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGG > NC_000913/361642‑361794 | aCCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTctc‑t > 1:77424/1‑81 (MQ=255) aTAGACAGGCGGTTCTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACtt < 1:77402/81‑1 (MQ=255) cAGGCGGTTCTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACTTCTgat > 2:77429/1‑81 (MQ=255) aGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTC‑TTATTTTCAACTTCTGATGGATGCGAGTGAtt > 1:77439/1‑80 (MQ=255) gTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACt < 1:77403/81‑1 (MQ=255) tttCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCa > 2:77437/1‑81 (MQ=255) cTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACAtt < 2:77404/81‑1 (MQ=255) ccGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTa < 2:77405/79‑1 (MQ=255) ccGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAAc < 2:77406/81‑1 (MQ=255) ggCAAACATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGttt > 1:77428/1‑81 (MQ=255) aaCATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTccc < 2:77407/81‑1 (MQ=255) aCATTTCTTTAATCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCa > 1:77427/1‑81 (MQ=255) tAATCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCtt > 1:77443/1‑81 (MQ=255) aaTCAGGCTTTCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCttt > 1:77458/1‑81 (MQ=255) tttCTCTTT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCgg > 1:77444/1‑80 (MQ=255) | ACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTC‑TT‑TTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGG > NC_000913/361642‑361794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |