| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,946,819 | 0 | A | T | 69.2% | 32.9 / 20.5 | 26 | E190V (GAA→GTA) | yebB | putative papain‑like amidase YebB |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base T (16/2); total (22/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTC > NC_000913/1946742‑1946897 | gTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTCTGGTGTTGATTCATGCGGAGGGAGTGGAAAc > 2:355635/1‑81 (MQ=255) tGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACg > 2:355612/1‑81 (MQ=255) tGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTCGGTGTTGATTCATGCGGAGGGAGTGGAAACg > 2:355614/1‑81 (MQ=255) gggAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGc > 2:355611/1‑81 (MQ=255) gAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCt > 2:355615/1‑81 (MQ=255) aaaaCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCg > 2:355622/1‑81 (MQ=255) aaaCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCg > 2:355632/1‑80 (MQ=255) aaCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCgg > 1:355617/1‑80 (MQ=255) gTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACt > 2:355633/1‑80 (MQ=255) aCGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGAcc > 1:830783/1‑81 (MQ=255) agccagTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGc > 2:355608/1‑81 (MQ=255) gccagATTGTGGCATCAGCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCg > 2:355627/1‑81 (MQ=255) ccagTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCgg > 1:355613/1‑81 (MQ=255) ttGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATaa > 2:355618/1‑81 (MQ=255) ggCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACtt > 2:355638/1‑80 (MQ=255) ccGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTc > 1:355619/1‑81 (MQ=255) cGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTc < 1:355594/80‑1 (MQ=255) gTTTGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGccc < 2:355595/81‑1 (MQ=255) tGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCctct > 1:355634/1‑81 (MQ=255) tGGTGTTGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCctct > 1:355628/1‑81 (MQ=255) ttGATTCATGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGc > 1:355616/1‑79 (MQ=255) aTGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAg > 1:355620/1‑81 (MQ=255) tGCGGAGGGAGTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGc > 2:355625/1‑81 (MQ=255) ggagggagTGGTAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCcgc < 2:355596/81‑1 (MQ=255) agggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGa > 1:355624/1‑81 (MQ=255) gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTc < 2:355597/80‑1 (MQ=255) | GTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTC > NC_000913/1946742‑1946897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |