Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,506,257	C→T	A29V (GCG→GTG)	sutR →	DNA‑binding transcriptional dual regulator SutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,506,257	0	C	T	100.0%	89.6 / NA	25	A29V (GCG→GTG)	sutR	DNA‑binding transcriptional dual regulator SutR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

ATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGCGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTCGCGACATTATGGAA  >  NC_000913/1506179‑1506332                                                                               |                                                                            aTCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGa                                                                           >  2:285825/1‑81 (MQ=255) aTCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGa                                                                           <  1:285797/81‑1 (MQ=255)   ctcgctcgCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACg                                                                         >  1:285832/1‑81 (MQ=255)      gctcgctTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGAc                                                                          <  1:285798/77‑1 (MQ=255)          gctTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGt                                                                    <  2:285799/79‑1 (MQ=255)                         cacTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAg                                                   <  2:285800/81‑1 (MQ=255)                          acTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAgg                                                  <  2:285801/81‑1 (MQ=255)                          acTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGATTCTAATGCAATGTTAgg                                                  >  1:285834/1‑81 (MQ=255)                           cTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAggg                                                 <  1:285802/81‑1 (MQ=255)                              aaaCAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAggg                                                 >  2:285827/1‑78 (MQ=255)                                  aacTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTg                                          <  1:285803/81‑1 (MQ=255)                                        gCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGt                                     >  2:285824/1‑80 (MQ=255)                                              agcGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGagt                              >  1:285844/1‑81 (MQ=255)                                              agcGCGGCTGGAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGagt                              <  2:285804/81‑1 (MQ=255)                                                        gAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGAc                    >  1:285831/1‑81 (MQ=255)                                                        gAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGAc                    <  1:285806/81‑1 (MQ=255)                                                        gAGTCTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGAc                    <  1:285805/81‑1 (MQ=255)                                                            cTTTCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTc                >  1:285837/1‑81 (MQ=255)                                                              ttCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTcgc              >  1:285835/1‑81 (MQ=255)                                                               tCGCGACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTc                <  1:285807/78‑1 (MQ=255)                                                                cgcgACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTcgcg             <  2:285808/80‑1 (MQ=255)                                                                 gcgACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTCGCGa            >  1:285842/1‑80 (MQ=255)                                                                 gcgACTGGCAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTCGCGAc           <  2:285809/81‑1 (MQ=255)                                                                         cAGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTCGCGACATTATgg    >  2:285839/1‑80 (MQ=255)                                                                          aGAAGTGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTCGCGACATTATGGaa  <  2:285810/81‑1 (MQ=255)                                                                               |                                                                            ATCTCGCTCGCTTTTTATCCACCACACTTAAACAACTACGCCAGCAGCGCGGCTGGAGTCTTTCGCGACTGGCAGAAGCGACGGGCGTTTCTAAAGCAATGTTAGGGCAGATTGAGCGTAATGAGTCCAGTCCGACGGTCGCGACATTATGGAA  >  NC_000913/1506179‑1506332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑