Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,513,132	G→A	V106M (GTG→ATG)	ydcT →	putative ABC transporter ATP‑binding protein YdcT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,513,132	0	G	A	100.0%	85.7 / NA	24	V106M (GTG→ATG)	ydcT	putative ABC transporter ATP‑binding protein YdcT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/7);  total (17/7)

GTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGT  >  NC_000913/1513057‑1513206                                                                            |                                                                           gTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGaa                                                                        >  1:286919/1‑80 (MQ=255)  tCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAAt                                                                       <  1:286896/80‑1 (MQ=255)         ggACTACGCGCTATTTCCGCATATGTAGATTCTTGACAATGTCGCTTATGGGCTGATGGTCAAAGGCATGAATAAAAag                                                                 >  2:286926/1‑79 (MQ=255)               cgcgCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGc                                                          <  2:286897/80‑1 (MQ=255)                        tCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGgc                                                >  1:286922/1‑81 (MQ=255)                            cATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGgcgc                                              >  1:286944/1‑79 (MQ=255)                            cATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCaa                                            <  2:286898/81‑1 (MQ=255)                               atGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAagag                                         >  1:286942/1‑81 (MQ=255)                                  tCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGgcg                                      >  1:286939/1‑81 (MQ=255)                                    gATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCt                                    >  2:286929/1‑81 (MQ=255)                                    gATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCt                                    <  2:286899/81‑1 (MQ=255)                                     aTTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTg                                   >  1:286918/1‑81 (MQ=255)                                           gACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGgaga                               >  2:286924/1‑79 (MQ=255)                                                  tCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGt                      <  1:286900/81‑1 (MQ=255)                                                  tCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGt                      >  1:286932/1‑81 (MQ=255)                                                    gCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGtt                     <  1:286901/80‑1 (MQ=255)                                                        aTGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGt                >  2:286928/1‑81 (MQ=255)                                                           ggCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTg             >  2:286940/1‑81 (MQ=255)                                                             cTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCGCGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTa           >  2:286938/1‑81 (MQ=255)                                                              tGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTa           >  2:286943/1‑80 (MQ=255)                                                                aTGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTAc          <  1:286902/79‑1 (MQ=255)                                                                      aaaGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACg   >  1:286920/1‑80 (MQ=255)                                                                       aaGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGt  >  2:286930/1‑80 (MQ=255)                                                                       aaGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGt  >  2:286931/1‑80 (MQ=255)                                                                            |                                                                           GTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGT  >  NC_000913/1513057‑1513206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑