Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,542,678	T→C	Q461Q (CAA→CAG)	narU ←	nitrate/nitrite transporter NarU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,542,678	0	T	C	100.0%	88.5 / NA	25	Q461Q (CAA→CAG)	narU	nitrate/nitrite transporter NarU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

CCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCA  >  NC_000913/1542600‑1542754                                                                               |                                                                             ccTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTg                                                                            >  1:291833/1‑81 (MQ=255) ccTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTg                                                                            >  1:291838/1‑81 (MQ=255)     cTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCt                                                                         <  2:291793/80‑1 (MQ=255)         aGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACt                                                                    <  2:291794/81‑1 (MQ=255)                  ttGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGAc                                                           <  2:291795/81‑1 (MQ=255)                    gggATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCa                                                         >  1:291835/1‑81 (MQ=255)                            aTGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAAccag                                                 >  2:291825/1‑81 (MQ=255)                                   gTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAgg                                            <  2:291796/79‑1 (MQ=255)                                   gTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGt                                           >  1:291831/1‑80 (MQ=255)                                    tCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTc                                          <  2:291797/80‑1 (MQ=255)                                           cGACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGcac                                  <  1:291798/81‑1 (MQ=255)                                            gACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGcaca                                 >  2:291834/1‑81 (MQ=255)                                            gACACGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGcaca                                 >  1:291827/1‑81 (MQ=255)                                              cacGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGcacac                                >  1:291828/1‑80 (MQ=255)                                               acGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACaa                              <  2:291799/81‑1 (MQ=255)                                                cGGTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACaaa                             <  1:291800/81‑1 (MQ=255)                                                 ggTTGTACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAAc                            <  2:291801/81‑1 (MQ=255)                                                      tACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGt                       <  1:291803/81‑1 (MQ=255)                                                      tACATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGt                       <  1:291802/81‑1 (MQ=255)                                                        cATCAGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTag                     <  2:291804/81‑1 (MQ=255)                                                            aGGCATCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTag                     >  2:291839/1‑77 (MQ=255)                                                                 tCGATCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTaa             >  2:291846/1‑80 (MQ=255)                                                                    aTCTCTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTaaaaa          >  2:291837/1‑80 (MQ=255)                                                                     tctcTTATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAaca        >  1:291847/1‑81 (MQ=255)                                                                         ttATTTCTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAacac       <  1:291805/78‑1 (MQ=255)                                                                            tttctGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCa  <  1:291806/80‑1 (MQ=255)                                                                               |                                                                             CCTGCTCCAGGAATGAATTGGGATCTGATGCCTTGTCGGATGCGACACGGTTGTACATCAGGCATCGATCTCTTATTTTTGGCTGAACTTCCGCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCA  >  NC_000913/1542600‑1542754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑