Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,549,287	C→A	S629S (TCC→TCA)	fdnG →	formate dehydrogenase N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,549,287	0	C	A	100.0%	107.3 / NA	30	S629S (TCC→TCA)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/16);  total (14/16)

TCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCCGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTG  >  NC_000913/1549209‑1549359                                                                               |                                                                         tCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAgg                                                                        >  2:292721/1‑81 (MQ=255)  ccGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGt                                                                       >  2:292703/1‑81 (MQ=255)   cGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGt                                                                       <  1:292675/80‑1 (MQ=255)   cGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTc                                                                      <  2:292676/81‑1 (MQ=255)     gCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGc                                                                    <  1:292677/81‑1 (MQ=255)        tCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTgg                                                                 >  1:292720/1‑81 (MQ=255)             tCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTg                                                              >  2:292728/1‑79 (MQ=255)             tCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTg                                                              >  2:292711/1‑79 (MQ=255)               aGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGt                                                          >  2:292715/1‑81 (MQ=255)                  cTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGc                                                       <  1:292678/81‑1 (MQ=255)                      aGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTg                                                   >  2:292716/1‑81 (MQ=255)                                  gCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGa                                       >  2:292713/1‑81 (MQ=255)                                   ccTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGAc                                      <  2:292679/81‑1 (MQ=255)                                    cTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGAcg                                     <  1:292680/81‑1 (MQ=255)                                              cTTTGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGa                           <  2:292681/81‑1 (MQ=255)                                               tttGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGcc                            >  2:292723/1‑78 (MQ=255)                                                 tGCTGAAGAAGATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGaa                          <  1:292682/79‑1 (MQ=255)                                                      aagaagATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGcgcg                     <  2:292683/79‑1 (MQ=255)                                                      aagaagATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGt                    >  1:292717/1‑80 (MQ=255)                                                      aagaagATGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGt                    >  1:292710/1‑80 (MQ=255)                                                       agaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTaa                  <  2:292684/81‑1 (MQ=255)                                                        gaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAac                 <  2:292685/81‑1 (MQ=255)                                                        gaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAac                 <  2:292686/81‑1 (MQ=255)                                                        gaagaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAac                 <  1:292687/81‑1 (MQ=255)                                                          agaTGGTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAacga               >  1:292718/1‑81 (MQ=255)                                                               gTTCTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGt           >  1:292719/1‑80 (MQ=255)                                                                  cTATCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGaaa       >  1:292732/1‑81 (MQ=255)                                                                     tCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGaaa       <  1:292688/78‑1 (MQ=255)                                                                     tCGCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAAt      <  2:292689/79‑1 (MQ=255)                                                                       gCTAACTCAGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTg  <  1:292690/81‑1 (MQ=255)                                                                               |                                                                         TCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCTGAAGAAGATGGTTCTATCGCTAACTCCGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAGGACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTG  >  NC_000913/1549209‑1549359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑