Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,612,952	G→A	S100S (TCC→TCT)	glsB ←	glutaminase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,612,952	0	G	A	100.0%	109.8 / NA	30	S100S (TCC→TCT)	glsB	glutaminase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/13);  total (17/13)

CGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAGGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGA  >  NC_000913/1612879‑1613028                                                                          |                                                                             cGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTg                                                                        >  2:302374/1‑80 (MQ=255)   cAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAAc                                                                     <  2:302340/81‑1 (MQ=255)     gACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACgg                                                                   >  1:302369/1‑81 (MQ=255)      accaccAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGt                                                                  >  1:302379/1‑81 (MQ=255)         accaGCGCACCGGCATTAATGAACGGATT‑CGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATc                                                              >  2:302385/1‑81 (MQ=255)            aGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCa                                                            <  1:302341/81‑1 (MQ=255)             gcgcACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAg                                                           <  1:302342/81‑1 (MQ=255)                  ccGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGa                                                      <  2:302343/81‑1 (MQ=255)                        ttAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTg                                                <  2:302345/81‑1 (MQ=255)                        ttAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTg                                                <  1:302344/81‑1 (MQ=255)                          aaTGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGAAGGATCTTTGcc                                              >  2:302386/1‑81 (MQ=255)                           aTGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGcc                                              <  1:302346/80‑1 (MQ=255)                             gAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAc                                           >  2:302384/1‑81 (MQ=255)                              aaCGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTTAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcg                                          <  1:302347/81‑1 (MQ=255)                              aaCGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcg                                          >  2:302382/1‑81 (MQ=255)                              aaCGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcg                                          >  2:302375/1‑81 (MQ=255)                                  gATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGtt                                      >  1:302388/1‑81 (MQ=255)                                  gATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGtt                                      >  1:302387/1‑81 (MQ=255)                                   aTTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTg                                     >  1:302377/1‑81 (MQ=255)                                     tACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGc                                    >  1:302376/1‑80 (MQ=255)                                                cccTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCctct                        <  1:302348/81‑1 (MQ=255)                                                    gCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg                     >  1:302389/1‑80 (MQ=255)                                                     cTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg                     <  1:302349/79‑1 (MQ=255)                                                      tCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAg                  >  1:302392/1‑81 (MQ=255)                                                            tCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATg             <  1:302350/80‑1 (MQ=255)                                                            tCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGa            >  2:302383/1‑81 (MQ=255)                                                              cAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACg          >  2:302399/1‑81 (MQ=255)                                                                 ttGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCAt       <  1:302352/81‑1 (MQ=255)                                                                 ttGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCAt       <  1:302351/81‑1 (MQ=255)                                                                       tAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGa  >  2:302391/1‑80 (MQ=255)                                                                          |                                                                             CGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAGGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGA  >  NC_000913/1612879‑1613028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑