Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	215,261	A→T	intergenic (+6/‑8)	arfB → / → nlpE	peptidyl‑tRNA hydrolase, ribosome rescue factor/lipoprotein NlpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	215,261	0	A	T	100.0%	123.3 / NA	34	intergenic (+6/‑8)	arfB/nlpE	peptidyl‑tRNA hydrolase, ribosome rescue factor/lipoprotein NlpE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/16);  total (18/16)

AGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGATGTAATAA  >  NC_000913/215184‑215336                                                                              |                                                                            aGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATgg                                                                           >  1:47706/1‑80 (MQ=255)  ggCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGaa                                                                         <  1:47679/81‑1 (MQ=255)   gCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAat                                                                        >  1:47710/1‑81 (MQ=255)   gCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAat                                                                        <  2:47680/81‑1 (MQ=255)         aTCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAatggatgg                                                                  <  1:47681/81‑1 (MQ=255)            gAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTg                                                                <  1:47684/80‑1 (MQ=255)            gAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTg                                                                <  2:47683/80‑1 (MQ=255)            gAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTg                                                                <  2:47682/80‑1 (MQ=255)              aaGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGaa                                                              >  2:47740/1‑80 (MQ=255)                 cacaAAAAGCAAGCGTGAAGGCGATGCGCGGCAAGGTGGGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGaaaaaa                                                          <  1:47685/81‑1 (MQ=255)                  acaAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAg                                                         >  2:47744/1‑81 (MQ=255)                      aaaTCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGAt                                                     <  1:47686/81‑1 (MQ=255)                      aaaTCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGAt                                                     <  1:47687/81‑1 (MQ=255)                       aaTCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATa                                                    <  2:47688/81‑1 (MQ=255)                        aTCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAg                                                   >  1:47730/1‑81 (MQ=255)                          cAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGt                                                  >  2:47721/1‑80 (MQ=255)                             gCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGa                                                >  2:47737/1‑79 (MQ=255)                                 gAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGa                                          >  2:47736/1‑81 (MQ=255)                                  aaGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGAt                                         <  2:47689/81‑1 (MQ=255)                                  aaGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGAt                                         >  1:47711/1‑81 (MQ=255)                                    ggCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATgg                                       >  2:47747/1‑81 (MQ=255)                                      cGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCt                                     <  2:47690/81‑1 (MQ=255)                                       gATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTg                                    >  2:47731/1‑81 (MQ=255)                                              ggCAAAGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg                             <  2:47691/81‑1 (MQ=255)                                                 aaaGTGCGCAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCct                          >  2:47753/1‑81 (MQ=255)                                                       cgcAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTAc                    >  2:47757/1‑81 (MQ=255)                                                         cAGCGGTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTActc                  >  2:47738/1‑81 (MQ=255)                                                             ggTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGAt              >  2:47739/1‑81 (MQ=255)                                                             ggTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATAGCTGTAATCAGCCTCTTTACTCTGAt              <  1:47692/81‑1 (MQ=255)                                                              gTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATg             >  2:47745/1‑81 (MQ=255)                                                              gTCGGGAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCCGATg             <  1:47693/81‑1 (MQ=255)                                                                 gggAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGa          >  2:47756/1‑81 (MQ=255)                                                                  ggAATAAAAAGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGAt         <  1:47694/81‑1 (MQ=255)                                                                         aaaGATGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGATGtaataa  >  1:47742/1‑81 (MQ=255)                                                                              |                                                                            AGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGATGTAATAA  >  NC_000913/215184‑215336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑