Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,615,877	A→T	T39S (ACA→TCA)	yneK →	protein YneK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,615,877	0	A	T	100.0%	31.3 / NA	10	T39S (ACA→TCA)	yneK	protein YneK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (7/3);  total (7/3)

TCCTGATGATTTTCCCGTACGTAATATTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTACAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCT  >  NC_000913/1615801‑1615950                                                                             |                                                                          tCCTGATGATTTTCCCGTACGTAATATTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCaa                                                                        >  1:302868/1‑80 (MQ=255)                        atatTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGgcgc                                                 <  2:302855/80‑1 (MQ=255)                          atTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACg                                              >  2:302879/1‑81 (MQ=255)                               aCCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCttt                                         <  1:302856/81‑1 (MQ=255)                                        aaaaGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCaa                                >  2:302867/1‑81 (MQ=255)                                         aaaGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAAc                               >  2:302869/1‑81 (MQ=255)                                           agaagTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTc                             <  1:302857/81‑1 (MQ=255)                                                    aaaGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACaa                     >  1:302871/1‑80 (MQ=255)                                                                tGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTtaa        >  2:302875/1‑81 (MQ=255)                                                                      aCTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCt  >  2:302874/1‑81 (MQ=255)                                                                             |                                                                          TCCTGATGATTTTCCCGTACGTAATATTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTACAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCT  >  NC_000913/1615801‑1615950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑