Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,621,770	A→G	I147V (ATC→GTC)	ydeE →	dipeptide exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,621,770	0	A	G	100.0%	79.5 / NA	23	I147V (ATC→GTC)	ydeE	dipeptide exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

TTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTACACCATGCTAAACATTGGCTGGACCATCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGC  >  NC_000913/1621692‑1621846                                                                               |                                                                             tttGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTACACCATGCTAAACATTGGCTGGACCGTc                                                                            >  1:303729/1‑81 (MQ=255)     ccGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTACACCATGCTAAACATTGGCTGGACCGTCGGTc                                                                        >  1:303724/1‑81 (MQ=255)     ccGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTACACCATGCTAAACATTGGCTGGACCGTCGGTc                                                                        >  1:303728/1‑81 (MQ=255)         cAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTACACCATGCTAAACATTGGCTGGACCGTCGGTccgcc                                                                    <  1:303689/81‑1 (MQ=255)                       ccaGCAAAACGAAAATCTTCTCAATCAACTACACCATGCTAAACATTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGt                                                      >  1:303732/1‑81 (MQ=255)                               aCGAAAATCTTCTCAATCAACTACACCATGCTAAACATTGGCTGGACCGTCGGTCGGCCGCTCTGCACGCTGTTGGTAATg                                              >  1:303727/1‑81 (MQ=255)                               aCGAAAATCTTCTCAATCAACTACACCATGCTAAACATTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATg                                              >  1:303718/1‑81 (MQ=255)                                             aatcaaCTACACCATGCTAAACATTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCaa                                   <  2:303690/78‑1 (MQ=255)                                               tcaaCTACACCATGCTAAACATTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGc                              <  2:303691/81‑1 (MQ=255)                                                caaCTACACCATGCTAAACATTGGCTGGACCGTCGGTCCGCCGCTCGGCTCGCTGTTGGTAATGCAGAGCATCAATCTGcc                             >  2:303734/1‑81 (MQ=255)                                                   cTACACCATGCTAAACATTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCtt                          >  2:303737/1‑81 (MQ=255)                                                      cacCATGCTAAACATTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTct                        <  2:303692/80‑1 (MQ=255)                                                         cATGCTAAACATTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTctggct                    <  2:303693/81‑1 (MQ=255)                                                          aTGCTAAACATTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTctggctg                   >  1:303726/1‑81 (MQ=255)                                                                 aCATTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTa            >  1:303733/1‑81 (MQ=255)                                                                   aTTGGCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATc          <  2:303694/81‑1 (MQ=255)                                                                      ggCTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTg        <  1:303695/80‑1 (MQ=255)                                                                        cTGGACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTc     <  2:303696/81‑1 (MQ=255)                                                                           gACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTcc    <  1:303699/79‑1 (MQ=255)                                                                           gACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTcc    <  2:303697/79‑1 (MQ=255)                                                                           gACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCcgc  >  1:558253/1‑81 (MQ=255)                                                                           gACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCcgc  <  1:303698/81‑1 (MQ=255)                                                                           gACCGTCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCcg   <  2:303700/80‑1 (MQ=255)                                                                               |                                                                             TTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTACACCATGCTAAACATTGGCTGGACCATCGGTCCGCCGCTCGGCACGCTGTTGGTAATGCAGAGCATCAATCTGCCCTTCTGGCTGGCAGCTATCTGTTCCGC  >  NC_000913/1621692‑1621846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑