Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,671,696	T→C	intergenic (+12/‑81)	asr → / → ydgU	acid shock protein/uncharacterized protein YdgU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,671,696	0	T	C	100.0%	29.9 / NA	10	intergenic (+12/‑81)	asr/ydgU	acid shock protein/uncharacterized protein YdgU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/5);  total (5/5)

AAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGG  >  NC_000913/1671622‑1671771                                                                           |                                                                            aaaCACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCt                                                                       <  2:311376/81‑1 (MQ=255)       gCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGgcgcg                                                                 >  1:310787/1‑81 (MQ=255)           aGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGcccc                                                             <  1:310764/81‑1 (MQ=255)                  caGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCgcgc                                                      <  1:310765/81‑1 (MQ=255)                    gCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCt                                                    >  2:310785/1‑81 (MQ=255)                                    aaaaCCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTa                                    <  1:310766/81‑1 (MQ=255)                                                              aaGTTTTCAACAGCAATGTTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCtt           >  2:310782/1‑80 (MQ=255)                                                                gTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAg        >  1:310784/1‑81 (MQ=255)                                                                gTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAg        <  2:310767/81‑1 (MQ=255)                                                                      aaCAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAgg  >  1:310788/1‑81 (MQ=255)                                                                           |                                                                            AAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGG  >  NC_000913/1671622‑1671771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑