Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,706,877	A→T	D177V (GAT→GTT)	rsxB →	SoxR [2Fe‑2S] reducing system protein RsxB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,706,877	0	A	T	100.0%	103.1 / NA	29	D177V (GAT→GTT)	rsxB	SoxR [2Fe‑2S] reducing system protein RsxB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/15);  total (14/15)

GTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAAT  >  NC_000913/1706807‑1706951                                                                       |                                                                           gtTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAacac                                                                   >  2:316285/1‑80 (MQ=255)  ttGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACAcc                                                                  <  2:316244/80‑1 (MQ=255)     aTCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTc                                                              <  1:316245/81‑1 (MQ=255)         gTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGt                                                          <  2:316246/81‑1 (MQ=255)           gCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGc                                                        >  2:316290/1‑81 (MQ=255)                    aCTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTc                                               <  1:316247/81‑1 (MQ=255)                     cTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTcc                                              <  1:316248/81‑1 (MQ=255)                     cTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTcc                                              >  1:316274/1‑81 (MQ=255)                     cTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTcc                                              >  2:316281/1‑81 (MQ=255)                        cATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCg                                            <  1:316250/80‑1 (MQ=255)                        cATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGt                                           <  2:316249/81‑1 (MQ=255)                          tctcGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTg                                          >  1:316278/1‑80 (MQ=255)                              gTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACTCCATTCCCGTGCGTATCATTCCCGTGGAaca                                     >  2:316280/1‑81 (MQ=255)                                tGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAacac                                    >  2:316282/1‑80 (MQ=255)                                 gCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCa                                  >  1:316284/1‑81 (MQ=255)                                   aaCCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATg                                <  1:316251/81‑1 (MQ=255)                                    aCCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGc                               >  1:316277/1‑81 (MQ=255)                                     ccGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCt                              <  1:316253/81‑1 (MQ=255)                                     ccGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTAACATTCCCGTGGAACACCATGCt                              <  2:316252/81‑1 (MQ=255)                                      cGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCtt                             <  1:316254/81‑1 (MQ=255)                                        gTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTaa                           <  1:316255/81‑1 (MQ=255)                                                aaCACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATtct                   >  1:316283/1‑81 (MQ=255)                                                  cacCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTctct                 <  2:316256/81‑1 (MQ=255)                                                   acCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTg                >  2:316287/1‑81 (MQ=255)                                                    ccTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGc               <  2:316257/81‑1 (MQ=255)                                                        aCTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCAtt            <  1:316258/80‑1 (MQ=255)                                                         cTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCAtt            >  1:316296/1‑79 (MQ=255)                                                               gAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGaaaa     >  1:316286/1‑80 (MQ=255)                                                                  aTGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAaaaat  >  2:316279/1‑80 (MQ=255)                                                                       |                                                                           GTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAAT  >  NC_000913/1706807‑1706951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑