Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,722,167	C→T	A16V (GCC→GTC)	ydhK →	putative transporter YdhK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,722,167	0	C	T	100.0%	88.0 / NA	25	A16V (GCC→GTC)	ydhK	putative transporter YdhK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/11);  total (14/11)

ACCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGCCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTA  >  NC_000913/1722095‑1722239                                                                         |                                                                         accTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTgg                                                                  >  2:318703/1‑81 (MQ=255)  ccTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGgc                                                                 <  1:318656/81‑1 (MQ=255)   cTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGgcg                                                                >  1:318700/1‑81 (MQ=255)      tAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCaa                                                             >  1:318708/1‑81 (MQ=255)        gCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATg                                                           >  1:318715/1‑81 (MQ=255)          aTTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGc                                                         <  1:318658/81‑1 (MQ=255)          aTTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGc                                                         <  2:318657/81‑1 (MQ=255)                 tAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGc                                                  >  2:318720/1‑81 (MQ=255)                 tAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGc                                                  >  2:318707/1‑81 (MQ=255)                      cAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTAc                                             >  2:318694/1‑81 (MQ=255)                                   aTCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGc                                >  1:318712/1‑81 (MQ=255)                                      gTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCAt                             >  1:318704/1‑81 (MQ=255)                                       tCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCAtg                            <  1:318659/81‑1 (MQ=255)                                         aTGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCAtgtg                          >  1:318701/1‑81 (MQ=255)                                         aTGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCAtgt                           <  1:318660/80‑1 (MQ=255)                                         aTGGTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCAtgt                           <  2:318661/80‑1 (MQ=255)                                            gTCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCt                       <  2:318662/81‑1 (MQ=255)                                             tCCTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTg                      <  1:318663/81‑1 (MQ=255)                                              ccTTGCGCAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTgg                     >  2:318698/1‑81 (MQ=255)                                                   cgcAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTGGCGCTg                <  2:318664/81‑1 (MQ=255)                                                     cAATTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTGGCGCTGAc              <  2:318665/81‑1 (MQ=255)                                                      aaTTTGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTGGCGCTGACg             >  2:318710/1‑81 (MQ=255)                                                        tttGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTGGCGCTGACGGt           <  1:318666/81‑1 (MQ=255)                                                         ttGCCCTGGTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTGGCGCTGACGGtt          >  2:318718/1‑81 (MQ=255)                                                                 gTTCAGGGTCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTGGCGCTGACGGTTGCCtatta  >  2:318706/1‑81 (MQ=255)                                                                         |                                                                         ACCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGCCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTA  >  NC_000913/1722095‑1722239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑