Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,728,574	C→T	A76A (GCC→GCT)	rnt →	RNase T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,728,574	0	C	T	100.0%	56.6 / NA	17	A76A (GCC→GCT)	rnt	RNase T
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/9);  total (8/9)

CTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTT  >  NC_000913/1728496‑1728645                                                                               |                                                                        ctggctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCttt                                                                       <  1:319756/81‑1 (MQ=255)   ggctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTc                                                                      >  2:319791/1‑80 (MQ=255)      tgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACg                                                                  >  1:585919/1‑81 (MQ=255)       gATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCATATTTGCAACCAGAAGCCCTCGCTTTCAACgg                                                                 >  2:319785/1‑81 (MQ=255)        aTGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAATTTTGCAACCAGAAGCCCTCGCTTTCAACgg                                                                 >  2:319787/1‑80 (MQ=255)            cGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCAtt                                                             >  1:319790/1‑80 (MQ=255)               acacCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGAcc                                                         <  2:319757/81‑1 (MQ=255)                   cacaTTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGaa                                                     <  2:319758/81‑1 (MQ=255)                         acattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATc                                                <  1:319759/80‑1 (MQ=255)                                cACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATcgc                                        <  1:319760/81‑1 (MQ=255)                                 aCGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATcgcg                                       >  1:319794/1‑81 (MQ=255)                                                   gcgcAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACg                     <  1:319761/81‑1 (MQ=255)                                                     gcAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAg                   <  2:319763/81‑1 (MQ=255)                                                     gcAAATTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAg                   <  1:319762/81‑1 (MQ=255)                                                        aaTTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGgcg                >  2:319793/1‑81 (MQ=255)                                                         aTTTGCAACCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGgcgc               <  1:319764/81‑1 (MQ=255)                                                               aaCCAGAAGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCAc          >  2:319795/1‑80 (MQ=255)                                                                      aGCCCTCGCTTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAAtttt  <  2:319765/81‑1 (MQ=255)                                                                               |                                                                        CTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTT  >  NC_000913/1728496‑1728645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑