Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,730,699	C→T	A538V (GCT→GTT)	lhr →	putative ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,730,699	0	C	T	100.0%	100.3 / NA	28	A538V (GCT→GTT)	lhr	putative ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/20);  total (8/20)

CCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGCTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAG  >  NC_000913/1730621‑1730776                                                                               |                                                                              ccTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTc                                                                             <  1:320161/81‑1 (MQ=255)       gCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAAtt                                                                         <  1:320162/79‑1 (MQ=255)          aTTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTgg                                                                    >  2:320201/1‑81 (MQ=255)          aTTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTgg                                                                    >  1:320207/1‑81 (MQ=255)           tttttCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGc                                                                   <  2:320164/81‑1 (MQ=255)             tttCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCgg                                                                 >  1:320202/1‑81 (MQ=255)              ttCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCgg                                                                 <  2:320165/80‑1 (MQ=255)               tCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGt                                                                >  1:320208/1‑80 (MQ=255)                      ttCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgc                                                         <  1:320166/80‑1 (MQ=255)                         gCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgcggc                                                      <  1:921047/80‑1 (MQ=255)                         gCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgcggc                                                      <  2:320168/80‑1 (MQ=255)                         gCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAgcggc                                                      <  1:320167/80‑1 (MQ=255)                             cAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCAc                                                 <  2:320169/81‑1 (MQ=255)                                     tCTGGAACAGGGAGACCGGGATATTGACCGCCCGATCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCgg                                         <  2:320171/81‑1 (MQ=255)                                     tCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCgg                                         <  2:320170/81‑1 (MQ=255)                                         gAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATc                                      <  2:320173/80‑1 (MQ=255)                                         gAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATc                                      <  2:320172/80‑1 (MQ=255)                                           aCAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTg                                   <  1:320174/81‑1 (MQ=255)                                            cAGGGAGACCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTgg                                  >  1:320214/1‑81 (MQ=255)                                                gagaCCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATg                              >  2:320218/1‑81 (MQ=255)                                                 agaCCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATg                              >  1:320210/1‑80 (MQ=255)                                                   aCCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGtat                           <  2:320175/81‑1 (MQ=255)                                                   aCCGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGtat                           <  2:320176/81‑1 (MQ=255)                                                    ccGGGATATTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGtata                          <  1:320177/81‑1 (MQ=255)                                                         atatTGACCGCCCGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCgtg                     <  1:320178/81‑1 (MQ=255)                                                                  gCCCGACCTGGCGTTCAATTGTTGTCGGTTACCAACATCGGCACCATTCCTGATCGTGTCATGTATAGCGTGTTATTAccc            <  2:320179/81‑1 (MQ=255)                                                                     cGACCTGGCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGaa         <  1:320180/81‑1 (MQ=255)                                                                           ggCGTTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTg     >  2:320216/1‑79 (MQ=255)                                                                            gcgtTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTgaag  <  1:320181/81‑1 (MQ=255)                                                                               |                                                                              CCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGCGCTCAATTGTTGGCGGTTACCAGCGGCGGCACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAG  >  NC_000913/1730621‑1730776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑