Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	218,480	C→T	R99H (CGT→CAT)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	218,480	0	C	T	100.0%	139.9 / NA	38	R99H (CGT→CAT)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/17);  total (21/17)

AAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCACGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACC  >  NC_000913/218403‑218557                                                                              |                                                                              aaGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGt                                                                            >  1:48488/1‑81 (MQ=255)   gagCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTca                                                                          <  1:48418/81‑1 (MQ=255)   gagCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTc                                                                           >  1:48467/1‑80 (MQ=255)    agCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTcaa                                                                         <  2:48419/81‑1 (MQ=255)    agCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTcaa                                                                         >  2:48486/1‑81 (MQ=255)         aGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACaaaa                                                                    >  2:48478/1‑81 (MQ=255)              gTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACgcag                                                               <  2:48420/81‑1 (MQ=255)               ttACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACgcagc                                                              <  1:48421/81‑1 (MQ=255)                    aaTCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTc                                                         <  2:48422/81‑1 (MQ=255)                             aGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGt                                                 <  2:48423/80‑1 (MQ=255)                             aGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTa                                                >  1:48481/1‑81 (MQ=255)                             aGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTa                                                >  1:48490/1‑81 (MQ=255)                              gTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTAc                                               >  2:48477/1‑81 (MQ=255)                              gTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTAc                                               >  1:48482/1‑81 (MQ=255)                                  tAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGtt                                           <  2:48424/81‑1 (MQ=255)                                   aaCTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTc                                          >  1:48493/1‑81 (MQ=255)                                    aCTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTc                                          <  1:48425/80‑1 (MQ=255)                                        cttcATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCa                                       <  1:48426/79‑1 (MQ=255)                                        cttcATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAAc                                     >  1:110168/1‑81 (MQ=255)                                          tcATGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGa                                   >  1:48494/1‑81 (MQ=255)                                            aTGAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGAc                                  >  2:48485/1‑80 (MQ=255)                                              gAGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCAc                               >  1:48489/1‑81 (MQ=255)                                               aGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCAct                              <  1:48428/81‑1 (MQ=255)                                               aGTTGGGCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCAct                              <  2:48427/81‑1 (MQ=255)                                                   gggCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCActctct                          >  1:48499/1‑81 (MQ=255)                                                    ggCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCtt                         <  1:48429/81‑1 (MQ=255)                                                     gCCGAGTACGAACGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTg                        <  2:48430/81‑1 (MQ=255)                                                           tACGAACGGACGCTCGCCATGGTAAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACaa                  <  2:48431/81‑1 (MQ=255)                                                               aaCGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCg              >  1:48484/1‑81 (MQ=255)                                                               aaCGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCg              >  1:48432/1‑81 (MQ=255)                                                                aCGGACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCgg             <  2:48433/81‑1 (MQ=255)                                                                   gACGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCggctg          >  2:48507/1‑81 (MQ=255)                                                                    aCGCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCggctgg         >  1:48503/1‑81 (MQ=255)                                                                      gCTCGCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCggctggct       >  2:48487/1‑81 (MQ=255)                                                                          gCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGac    >  1:48491/1‑79 (MQ=255)                                                                          gCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGa     <  2:48434/79‑1 (MQ=255)                                                                          gCCATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAac   >  1:48521/1‑81 (MQ=255)                                                                           ccATGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAacc  <  2:48435/81‑1 (MQ=255)                                                                              |                                                                              AAGAGCTAAGCTCGTTACGAATCAGGTCAGTGATAACTTCTTCATGAGTTGGGCCGAGTACGAACGGACGCTCGCCACGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACC  >  NC_000913/218403‑218557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑