Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,556	A→G	H238R (CAT→CGT)	purR →	DNA‑binding transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,556	0	A	G	100.0%	88.3 / NA	26	H238R (CAT→CGT)	purR	DNA‑binding transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

CGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGC  >  NC_000913/1738478‑1738633                                                                               |                                                                              cGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTc                                                                             <  2:321502/81‑1 (MQ=255)      aGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGccc                                                                         >  1:321545/1‑80 (MQ=255)       gCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTa                                                                       >  1:321558/1‑81 (MQ=255)       gCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTa                                                                       >  1:321544/1‑81 (MQ=255)        cTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTAc                                                                      <  1:321503/81‑1 (MQ=255)          ggATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTg                                                                    >  1:321562/1‑81 (MQ=255)           gATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTg                                                                    <  2:321504/80‑1 (MQ=255)                     ggTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCtgt                                                         <  1:321505/81‑1 (MQ=255)                             tGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGc                                                   <  1:321506/79‑1 (MQ=255)                             tGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCg                                                  <  1:321507/80‑1 (MQ=255)                               aaCCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGat                                                <  2:321508/80‑1 (MQ=255)                                     aaTCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATgg                                         >  2:321552/1‑81 (MQ=255)                                     aaTCCGGTTATCGCGACATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATgg                                         <  2:321509/81‑1 (MQ=255)                                                cgcgCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCa                              >  1:486749/1‑81 (MQ=255)                                                     cATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtg                         <  1:321510/81‑1 (MQ=255)                                                     cATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtg                         >  2:321564/1‑81 (MQ=255)                                                      aTGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtgt                        <  2:321511/81‑1 (MQ=255)                                                      aTGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtgt                        <  2:321512/81‑1 (MQ=255)                                                         cagcaAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGtgc                      <  2:321513/80‑1 (MQ=255)                                                          agcaAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGtgctg                    >  2:321555/1‑81 (MQ=255)                                                            caAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTgctgct                  <  2:321514/81‑1 (MQ=255)                                                             aaaTCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTgctgct                  >  1:321571/1‑80 (MQ=255)                                                             aaaTCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTgctgct                  >  1:321557/1‑80 (MQ=255)                                                                  cTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCtgatga             >  2:321560/1‑80 (MQ=255)                                                                     tCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGaaa           <  1:321515/79‑1 (MQ=255)                                                                         aGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGcc     >  1:321565/1‑81 (MQ=255)                                                                            cgcgTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTgc  <  2:321516/81‑1 (MQ=255)                                                                            cgcgTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTgc  >  2:321569/1‑81 (MQ=255)                                                                               |                                                                              CGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGC  >  NC_000913/1738478‑1738633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑