Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,748,585	T→A	R485R (CGT→CGA)	ydhS →	FAD/NAD(P) binding domain‑containing protein YdhS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,748,585	0	T	A	100.0%	120.4 / NA	34	R485R (CGT→CGA)	ydhS	FAD/NAD(P) binding domain‑containing protein YdhS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/15);  total (19/15)

CGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTG  >  NC_000913/1748507‑1748658                                                                               |                                                                          cgAGCAATTACAGAAAACAGGGGATGAAGTCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAgg                                                                         <  2:323001/81‑1 (MQ=255)      aaTTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGcgcg                                                                    <  2:323002/81‑1 (MQ=255)       aTTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGt                                                                   <  1:323003/81‑1 (MQ=255)             gAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGtt                                                             >  1:323057/1‑81 (MQ=255)              aaaaCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTc                                                            <  1:323004/81‑1 (MQ=255)               aaaCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCg                                                           >  1:323049/1‑81 (MQ=255)               aaaCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTGCg                                                           >  2:323048/1‑81 (MQ=255)                 aCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGgc                                                         <  2:570477/81‑1 (MQ=255)                 aCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGgc                                                         <  1:323005/81‑1 (MQ=255)                   aGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGgcgc                                                       >  2:323044/1‑81 (MQ=255)                   aGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGgcgc                                                       >  1:323042/1‑81 (MQ=255)                      ggATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGtt                                                    <  2:323007/81‑1 (MQ=255)                      ggATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGtt                                                    <  1:323006/81‑1 (MQ=255)                           aaaTCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCt                                               <  2:323008/81‑1 (MQ=255)                              tCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGt                                            >  2:323052/1‑81 (MQ=255)                                ccTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGtt                                           >  1:323039/1‑80 (MQ=255)                                 cTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGa                                         >  2:323051/1‑81 (MQ=255)                                       ttGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCAc                                    >  1:323058/1‑80 (MQ=255)                                       ttGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCAc                                    <  2:323010/80‑1 (MQ=255)                                       ttGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACg                                   <  2:323009/81‑1 (MQ=255)                                        tGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGa                                  >  2:323041/1‑81 (MQ=255)                                         ggCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAc                                 >  2:323050/1‑81 (MQ=255)                                         ggCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAc                                 >  2:323061/1‑81 (MQ=255)                                            gAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAg                              <  1:323011/81‑1 (MQ=255)                                                 ttATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCttt                         <  2:323012/81‑1 (MQ=255)                                                   ataCGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTc                        <  2:323013/80‑1 (MQ=255)                                                    taCGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGt                      <  1:323014/81‑1 (MQ=255)                                                          aCAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAgg                 >  1:323059/1‑80 (MQ=255)                                                                   cGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACgg        >  1:323053/1‑80 (MQ=255)                                                                    gAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCa      >  2:323054/1‑81 (MQ=255)                                                                    gAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCa      >  2:323076/1‑81 (MQ=255)                                                                      aGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCAtg    >  1:323040/1‑81 (MQ=255)                                                                         tatTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCAtgtg  >  1:323063/1‑80 (MQ=255)                                                                          atTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCAtgtg  >  2:323045/1‑79 (MQ=255)                                                                               |                                                                          CGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTG  >  NC_000913/1748507‑1748658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑