Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,767,394	T→C	H431R (CAC→CGC)	ydiJ ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,767,394	0	T	C	100.0%	97.5 / NA	28	H431R (CAC→CGC)	ydiJ	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/18);  total (10/18)

TCGACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGTGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAC  >  NC_000913/1767316‑1767470                                                                               |                                                                             tCGACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGgcgt                                                                            <  1:325998/81‑1 (MQ=255)  cGACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGtt                                                                           >  1:326044/1‑81 (MQ=255)    aCGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTcc                                                                         <  2:325999/81‑1 (MQ=255)            gAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTAc                                                                 >  1:326046/1‑81 (MQ=255)             aaCATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACg                                                                <  2:326002/81‑1 (MQ=255)             aaCATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACg                                                                <  2:326001/81‑1 (MQ=255)             aaCATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACg                                                                <  2:326000/81‑1 (MQ=255)                    cGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTAt                                                         >  2:326058/1‑81 (MQ=255)                     gTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATc                                                        <  2:326003/81‑1 (MQ=255)                         cTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCa                                                    <  2:326004/81‑1 (MQ=255)                            aaGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGc                                                  <  1:326005/80‑1 (MQ=255)                                 gTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACg                                             >  1:326047/1‑80 (MQ=255)                                    gCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAAt                                         <  1:326006/81‑1 (MQ=255)                                     cTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAAtt                                        <  2:326007/81‑1 (MQ=255)                                          gAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCt                                    >  1:326048/1‑80 (MQ=255)                                             cagcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGTAGGTATCCTCAGCAAACGGAATTGGCTTAGc                                >  2:326054/1‑81 (MQ=255)                                             cagcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGc                                <  2:326008/81‑1 (MQ=255)                                              agcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCg                               <  2:326009/81‑1 (MQ=255)                                                 gcgcgAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCa                            <  1:326010/81‑1 (MQ=255)                                                    cgAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCt                         <  2:326011/81‑1 (MQ=255)                                                     gAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCtt                        >  2:326051/1‑81 (MQ=255)                                                         ttCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGc                    <  2:326012/81‑1 (MQ=255)                                                              cAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGc               <  2:326013/81‑1 (MQ=255)                                                                atatAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCa             <  1:326014/81‑1 (MQ=255)                                                                     aTCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAg           >  2:326057/1‑78 (MQ=255)                                                                         gCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCa    <  2:326015/81‑1 (MQ=255)                                                                          ccAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCaa   >  1:326053/1‑81 (MQ=255)                                                                          ccAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCAACTTTGGCATTGCCAAGCAGACCaa   <  2:326016/81‑1 (MQ=255)                                                                           cAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAc  >  2:326066/1‑81 (MQ=255)                                                                               |                                                                             TCGACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGTGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAC  >  NC_000913/1767316‑1767470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑