Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,789,895	C→T	L450L (TTG→TTA)	ydiU ←	UPF0061 family protein YdiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,789,895	0	C	T	100.0%	93.5 / NA	26	L450L (TTG→TTA)	ydiU	UPF0061 family protein YdiU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/15);  total (11/15)

CAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGT  >  NC_000913/1789818‑1789971                                                                              |                                                                             cagcTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAAc                                                                           <  1:329596/81‑1 (MQ=255) cagcTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAAc                                                                           >  2:329642/1‑81 (MQ=255)  agcTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACg                                                                          >  1:329640/1‑81 (MQ=255)        cTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCAt                                                                    <  2:329597/81‑1 (MQ=255)          tCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATg                                                                   >  2:329636/1‑80 (MQ=255)            cAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAg                                                                >  1:329638/1‑81 (MQ=255)             aGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAgg                                                               >  2:329645/1‑81 (MQ=255)                 gTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGt                                                           <  2:329598/81‑1 (MQ=255)                    tACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGc                                                         <  2:329599/80‑1 (MQ=255)                      ccccAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCGTCATGCAGGCGGTGCAAt                                                      >  2:329625/1‑81 (MQ=255)                              aGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCat                                              <  2:329600/81‑1 (MQ=255)                                   gacgacTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCAc                                         <  1:329601/81‑1 (MQ=255)                                   gacgacTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCAc                                         <  2:329602/81‑1 (MQ=255)                                           aCATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCt                                 <  2:329604/81‑1 (MQ=255)                                           aCATAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCt                                 <  1:329603/81‑1 (MQ=255)                                             aTAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGc                               >  1:329643/1‑81 (MQ=255)                                              tAGTCATCATCTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGcc                              <  2:329605/81‑1 (MQ=255)                                                   atcatcTCTGTCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCTCTTc                         <  1:329606/81‑1 (MQ=255)                                                             tCGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGt               <  1:329607/81‑1 (MQ=255)                                                              cGCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGtt              >  2:329652/1‑81 (MQ=255)                                                               gCTGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGtt              <  1:329608/80‑1 (MQ=255)                                                                 tGAAAGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTgcg           >  1:329648/1‑81 (MQ=255)                                                                   aaaGGATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTgcgc          >  2:329646/1‑80 (MQ=255)                                                                       gATTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAAcc     <  2:329609/81‑1 (MQ=255)                                                                        aTTTCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCa    <  1:329610/81‑1 (MQ=255)                                                                          ttCGTAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGt  >  2:329644/1‑81 (MQ=255)                                                                              |                                                                             CAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGT  >  NC_000913/1789818‑1789971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑