Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,831,779	C→T	A68A (GCG→GCA)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,831,779	0	C	T	100.0%	116.4 / NA	32	A68A (GCG→GCA)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/17);  total (15/17)

GCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTC  >  NC_000913/1831705‑1831856                                                                           |                                                                              gCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgt                                                                         <  2:336339/81‑1 (MQ=255)      gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt                                                                    >  1:336375/1‑81 (MQ=255)      gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt                                                                    <  2:336340/81‑1 (MQ=255)      gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt                                                                    >  2:336368/1‑81 (MQ=255)      gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt                                                                    >  2:336385/1‑81 (MQ=255)                   gCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACg                                                        <  2:336341/80‑1 (MQ=255)                     cAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAg                                                     >  2:336382/1‑81 (MQ=255)                          gaaGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTcc                                                 <  2:336343/78‑1 (MQ=255)                          gCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTcc                                                 <  2:336342/80‑1 (MQ=255)                          gCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCg                                                >  2:336398/1‑81 (MQ=255)                                ccGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATgc                                           <  1:336344/80‑1 (MQ=255)                                  ggCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCa                                        >  1:336380/1‑81 (MQ=255)                                       gTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATggtc                                   >  2:336388/1‑79 (MQ=255)                                        ttGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGccc                                  <  2:336345/81‑1 (MQ=255)                                          ggTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCa                                 <  2:336346/80‑1 (MQ=255)                                          ggTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAg                                >  2:336391/1‑81 (MQ=255)                                           gtgtAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAgc                               <  1:336347/81‑1 (MQ=255)                                             gtAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAgcgc                             >  2:336384/1‑81 (MQ=255)                                                aCCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGtt                          <  1:336348/81‑1 (MQ=255)                                                 ccGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTc                         <  2:336349/81‑1 (MQ=255)                                                   gTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCAc                       <  1:336350/81‑1 (MQ=255)                                                     tGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCg                     >  2:336386/1‑81 (MQ=255)                                                       ccGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCa                   <  1:336351/81‑1 (MQ=255)                                                         ggTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCTCAGCGCGTTCACCGCAAt                 >  1:336393/1‑81 (MQ=255)                                                         ggTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAAt                 >  1:336383/1‑81 (MQ=255)                                                           tATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGc               >  1:336396/1‑81 (MQ=255)                                                           tATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGc               <  2:336352/81‑1 (MQ=255)                                                           tATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGc               <  1:336353/81‑1 (MQ=255)                                                              gCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCAc            >  2:336399/1‑81 (MQ=255)                                                                   aaCTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCcgcg       <  2:336354/81‑1 (MQ=255)                                                                    acttacttGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCcgcg       >  1:336397/1‑80 (MQ=255)                                                                    acttacttGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGa      <  1:336355/81‑1 (MQ=255)                                                                        aCTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGt   <  1:336356/80‑1 (MQ=255)                                                                        aCTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTc  >  1:336387/1‑81 (MQ=255)                                                                           |                                                                              GCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTC  >  NC_000913/1831705‑1831856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑