Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,852,829	C→T	R384H (CGC→CAC)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,852,829	0	C	T	100.0%	77.3 / NA	22	R384H (CGC→CAC)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

TAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCA  >  NC_000913/1852753‑1852904                                                                             |                                                                            taatGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTaa                                                                         <  1:340003/81‑1 (MQ=255) taatGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTaa                                                                         <  2:340004/81‑1 (MQ=255)   atGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCGTTAACGAAACCCGAACCGCGCAGGTGTAAAt                                                                       >  1:340038/1‑81 (MQ=255)      ccGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCg                                                                    <  2:758462/81‑1 (MQ=255)        gCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGtt                                                                  >  2:340029/1‑81 (MQ=255)          aaCGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTgg                                                                >  1:340037/1‑81 (MQ=255)          aaCGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTgg                                                                <  2:340005/81‑1 (MQ=255)            cGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGc                                                               >  2:340043/1‑80 (MQ=255)              ccATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCaa                                                            <  2:340006/81‑1 (MQ=255)                 tAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGc                                                         <  2:340007/81‑1 (MQ=255)                 tAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGc                                                         <  2:340008/81‑1 (MQ=255)                           aGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGatat                                               <  1:340009/81‑1 (MQ=255)                              cTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATaaa                                            >  1:340036/1‑81 (MQ=255)                                 cgacgaTCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATAAAccg                                         >  2:340046/1‑81 (MQ=255)                                             cgACGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAg                              >  1:340041/1‑80 (MQ=255)                                               aCGGCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCaa                           <  2:340010/81‑1 (MQ=255)                                                 ggCATTAACGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAac                         <  2:340011/81‑1 (MQ=255)                                                         cGAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGt                 <  2:340012/81‑1 (MQ=255)                                                          gAAACCCGAACCGCGCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGta                >  2:340045/1‑81 (MQ=255)                                                                 gAACCGCGCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATaaa            >  1:340039/1‑78 (MQ=255)                                                                     cgcgCAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAagaaga     <  1:340013/81‑1 (MQ=255)                                                                        gcAGGTGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGatca  >  2:340035/1‑81 (MQ=255)                                                                             |                                                                            TAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCA  >  NC_000913/1852753‑1852904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑