Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,855,607	A→T	L122* (TTG→TAG)	ydjG ←	NADH‑dependent methylglyoxal reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,855,607	0	A	T	100.0%	105.7 / NA	29	L122* (TTG→TAG)	ydjG	NADH‑dependent methylglyoxal reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/12);  total (17/12)

GCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGA  >  NC_000913/1855529‑1855682                                                                               |                                                                            gCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAg                                                                           <  1:340391/81‑1 (MQ=255)   gATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCTATGTAATCAATACCCAGACGTTGCTAGCt                                                                         >  2:340436/1‑81 (MQ=255)   gATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCt                                                                         >  2:340429/1‑81 (MQ=255)     tCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTc                                                                        >  1:340392/1‑80 (MQ=255)      cGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCgc                                                                      >  2:340421/1‑81 (MQ=255)      cGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCgc                                                                      >  1:340423/1‑81 (MQ=255)       ggCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCgct                                                                     >  1:340428/1‑81 (MQ=255)                aaTGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACctct                                                            >  2:340435/1‑81 (MQ=255)                    gcggcACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTcgc                                                        >  1:340427/1‑81 (MQ=255)                        cACCGACTGCCAGTGCGTCATGTAGATATCTATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGa                                                     >  1:340439/1‑80 (MQ=255)                         aCCGACTGCCAGTGCGTCATGTAGATATCTATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGa                                                     >  2:340430/1‑79 (MQ=255)                          ccGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAg                                                  <  2:340393/81‑1 (MQ=255)                              cTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTc                                              <  1:340394/81‑1 (MQ=255)                               tGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTcc                                             >  2:340426/1‑81 (MQ=255)                                    gTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCgggg                                         >  1:340425/1‑80 (MQ=255)                                            tGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGtttt                                <  2:340395/81‑1 (MQ=255)                                                gATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTtata                            >  1:340434/1‑81 (MQ=255)                                                    tCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAAc                        <  1:340396/81‑1 (MQ=255)                                                            aTCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGAt                 <  2:340397/80‑1 (MQ=255)                                                             tCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCg               >  2:340398/1‑81 (MQ=255)                                                               aaTACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGcc             <  2:340401/81‑1 (MQ=255)                                                               aaTACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGcc             <  1:340399/81‑1 (MQ=255)                                                               aaTACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGc              >  2:340431/1‑80 (MQ=255)                                                               aaTACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGc              <  1:340400/80‑1 (MQ=255)                                                                   cccAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACt         >  1:340432/1‑81 (MQ=255)                                                                    ccAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACtt        <  2:340402/81‑1 (MQ=255)                                                                       gACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTttgt     <  1:340403/81‑1 (MQ=255)                                                                          gTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTttgttg   >  1:340438/1‑80 (MQ=255)                                                                          gTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGa  <  1:340404/81‑1 (MQ=255)                                                                               |                                                                            GCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGA  >  NC_000913/1855529‑1855682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑