| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 233,614 | C→T | M114I (ATG→ATA) | mltD ← | membrane‑bound lytic murein transglycosylase D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 233,614 | 0 | C | T | 100.0% | 69.1 / NA | 20 | M114I (ATG→ATA) | mltD | membrane‑bound lytic murein transglycosylase D |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/10); total (10/10) | |||||||||||
GCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCT > NC_000913/233536‑233691 | gCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTAt > 2:51585/1‑81 (MQ=255) ccTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTAt < 1:51552/80‑1 (MQ=255) ccTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTAt < 2:249115/80‑1 (MQ=255) ttGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGtttt > 1:51582/1‑81 (MQ=255) gcgcCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGtttttt < 2:51553/80‑1 (MQ=255) aGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTg > 2:51587/1‑81 (MQ=255) cGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGccc < 1:51554/81‑1 (MQ=255) gTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCt > 2:51581/1‑81 (MQ=255) ggATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAg < 1:51555/81‑1 (MQ=255) aaaaGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACat < 2:51556/81‑1 (MQ=255) aaaaGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACa > 2:51584/1‑80 (MQ=255) cACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGctct < 1:51558/79‑1 (MQ=255) cACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGctct < 1:51557/79‑1 (MQ=255) cACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGc > 2:51586/1‑81 (MQ=255) aTGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCg > 1:51593/1‑80 (MQ=255) ggTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTaaa < 2:51559/81‑1 (MQ=255) agtaCCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACa > 1:51602/1‑81 (MQ=255) aGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTg > 2:51599/1‑80 (MQ=255) ttCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGgag < 1:51560/81‑1 (MQ=255) aGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGct > 2:51591/1‑81 (MQ=255) | GCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCT > NC_000913/233536‑233691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |