Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,882,216	A→T	Q102L (CAG→CTG)	dmlA →	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,882,216	0	A	T	100.0%	102.1 / NA	28	Q102L (CAG→CTG)	dmlA	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/14);  total (14/14)

CGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATC  >  NC_000913/1882139‑1882292                                                                              |                                                                             cGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTa                                                                           >  1:344488/1‑81 (MQ=255)     ggCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGt                                                                        >  2:344474/1‑80 (MQ=255)     ggCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTc                                                                       <  1:344444/81‑1 (MQ=255)      gCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCa                                                                      <  2:344445/81‑1 (MQ=255)         ggCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAAcc                                                                   >  1:344489/1‑81 (MQ=255)          gCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAAcc                                                                   <  2:344446/80‑1 (MQ=255)                    gTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGtt                                                        >  2:344499/1‑81 (MQ=255)                     ttCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTc                                                       <  2:344447/81‑1 (MQ=255)                      tCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCg                                                      >  1:344482/1‑81 (MQ=255)                      tCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCg                                                      >  1:344485/1‑81 (MQ=255)                            ccATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCtt                                                <  1:344448/81‑1 (MQ=255)                                  ttCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTgg                                          >  2:344478/1‑81 (MQ=255)                                     gTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGt                                       >  1:344496/1‑81 (MQ=255)                                     gTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGt                                       <  2:344449/81‑1 (MQ=255)                                      ttGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGtt                                      <  2:344450/81‑1 (MQ=255)                                             gTTCGCTGCTGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGcc                               <  2:344451/81‑1 (MQ=255)                                                 gctgctGAAATTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGc                            <  1:344452/80‑1 (MQ=255)                                                        aaaTTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCggg                     <  2:344453/80‑1 (MQ=255)                                                          aTTTCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGaaa                  <  2:344454/81‑1 (MQ=255)                                                           tttCGTCGTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGaaaa                 <  2:344455/81‑1 (MQ=255)                                                             tcgtcgTGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAAc                <  2:344456/80‑1 (MQ=255)                                                               gtcgtGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGc             >  2:344505/1‑81 (MQ=255)                                                                  gtGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCt           <  1:344457/80‑1 (MQ=255)                                                                  gtGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTg          >  2:344512/1‑81 (MQ=255)                                                                  gtGAATTCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTg          >  1:344495/1‑81 (MQ=255)                                                                        tCGACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACa    >  1:344494/1‑81 (MQ=255)                                                                          gACCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATc  >  1:344493/1‑81 (MQ=255)                                                                           aCCTGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATc  >  1:344498/1‑80 (MQ=255)                                                                              |                                                                             CGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATC  >  NC_000913/1882139‑1882292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑