Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,900,559	C→A	E343* (GAA→TAA)	yoaE ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,900,559	0	C	A	100.0%	107.5 / NA	30	E343* (GAA→TAA)	yoaE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/20);  total (10/20)

GAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCC  >  NC_000913/1900483‑1900634                                                                             |                                                                            gAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATcc                                                                         <  2:347687/81‑1 (MQ=255) gAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATcc                                                                         <  2:347686/81‑1 (MQ=255)   aGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAg                                                                       <  1:347688/81‑1 (MQ=255)    gCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGt                                                                      <  1:347689/81‑1 (MQ=255)       aTCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCa                                                                   >  2:347747/1‑81 (MQ=255)          gccgccACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACcg                                                                <  1:347690/81‑1 (MQ=255)             gccACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCTCGa                                                             >  2:347749/1‑81 (MQ=255)                acaTCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACAt                                                          <  2:347691/81‑1 (MQ=255)                     aaCGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCg                                                      <  1:347692/80‑1 (MQ=255)                       cGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGa                                                   <  1:347693/81‑1 (MQ=255)                             ctTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGAc                                             >  1:347729/1‑81 (MQ=255)                                ccAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACt                                            <  2:347694/79‑1 (MQ=255)                                 cAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACtgtg                                         >  2:347731/1‑81 (MQ=255)                                 cAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACtgt                                          <  1:347695/80‑1 (MQ=255)                                   gcgcCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCg                                       <  1:347696/81‑1 (MQ=255)                                      ccaccaGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTg                                    <  2:347697/81‑1 (MQ=255)                                        accaGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGaa                                  <  2:347698/81‑1 (MQ=255)                                         ccggcagTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAag                                 <  2:347699/78‑1 (MQ=255)                                              agTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAagagag                             >  1:347739/1‑80 (MQ=255)                                                  cTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGtt                        >  2:347757/1‑81 (MQ=255)                                                      aGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCt                     <  2:347700/80‑1 (MQ=255)                                                      aGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTc                    >  1:347751/1‑81 (MQ=255)                                                      aGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTc                    >  2:347750/1‑81 (MQ=255)                                                        cACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTcgc                  <  2:347702/81‑1 (MQ=255)                                                        cACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTcgc                  <  1:347701/81‑1 (MQ=255)                                                             aCAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGAtt             >  2:347752/1‑81 (MQ=255)                                                              cAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGAtt             <  1:347703/80‑1 (MQ=255)                                                                 tACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCAt         <  1:347704/81‑1 (MQ=255)                                                                   ccGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATc        <  1:347705/80‑1 (MQ=255)                                                                        gatTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGAcccc  >  1:347760/1‑81 (MQ=255)                                                                             |                                                                            GAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCC  >  NC_000913/1900483‑1900634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑