Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,935,168	C→T	D383N (GAT→AAT)	zwf ←	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,935,168	0	C	T	100.0%	115.1 / NA	32	D383N (GAT→AAT)	zwf	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/15);  total (17/15)

AATAGCTCAGATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATT  >  NC_000913/1935090‑1935243                                                                               |                                                                            aaTAGCTCAGATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCa                                                                           >  1:353655/1‑81 (MQ=255)   tAGCTCAGATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACg                                                                         >  1:353662/1‑81 (MQ=255)       tCAGATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCtt                                                                     >  1:353659/1‑81 (MQ=255)         aGATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTtc                                                                    <  2:353621/80‑1 (MQ=255)          gATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTtcat                                                                  <  1:353623/81‑1 (MQ=255)          gATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTtcat                                                                  >  1:353624/1‑81 (MQ=255)          gATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAAGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTtcat                                                                  <  1:353622/81‑1 (MQ=255)             ccAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAg                                                               >  2:353656/1‑81 (MQ=255)             ccAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAg                                                               >  2:353654/1‑81 (MQ=255)              cAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAgg                                                              >  1:353661/1‑81 (MQ=255)                 cTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTg                                                           <  2:353625/81‑1 (MQ=255)                    ggTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTg                                                           >  2:353664/1‑78 (MQ=255)                    ggTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCa                                                         <  1:353626/80‑1 (MQ=255)                       gATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACg                                                     >  1:353668/1‑81 (MQ=255)                        aTTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACgg                                                    <  1:353627/81‑1 (MQ=255)                                      tttGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTAtt                                      <  2:353628/81‑1 (MQ=255)                                      tttGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTAt                                       <  2:353629/80‑1 (MQ=255)                                       ttGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTc                                     >  2:353647/1‑81 (MQ=255)                                           ggTCAAGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCg                                 >  1:353663/1‑81 (MQ=255)                                               aaGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAg                             <  2:353630/81‑1 (MQ=255)                                                aGGCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCgg                                >  2:353660/1‑77 (MQ=255)                                                  gCCAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATc                          <  2:353631/81‑1 (MQ=255)                                                   ccAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATcc                         >  2:353667/1‑81 (MQ=255)                                                    cAGGAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCt                        >  2:353671/1‑81 (MQ=255)                                                      ggAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGc                      <  1:353632/81‑1 (MQ=255)                                                       gAACTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGcc                     <  2:353633/81‑1 (MQ=255)                                                        aaCTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCa                    >  2:353675/1‑81 (MQ=255)                                                         aCTTTATTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCAc                   <  2:353634/81‑1 (MQ=255)                                                              aTTCAGTACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTc              >  2:353682/1‑81 (MQ=255)                                                                    tACCTGGATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTaaa        <  1:353635/81‑1 (MQ=255)                                                                          gATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGAtt  <  1:353636/81‑1 (MQ=255)                                                                          gATATTCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGAtt  >  2:353669/1‑81 (MQ=255)                                                                               |                                                                            AATAGCTCAGATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATT  >  NC_000913/1935090‑1935243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑