Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,942,614	A→G	intergenic (‑31/‑48)	znuA ← / → znuC	Zn(2(+)) ABC transporter periplasmic binding protein/Zn(2(+)) ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,942,614	0	A	G	100.0%	92.9 / NA	26	intergenic (‑31/‑48)	znuA/znuC	Zn(2(+)) ABC transporter periplasmic binding protein/Zn(2(+)) ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/11);  total (15/11)

GGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAA  >  NC_000913/1942542‑1942685                                                                         |                                                                        ggcggATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGa                                                                  >  1:354984/1‑80 (MQ=255) ggcggATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGa                                                                  >  1:354987/1‑80 (MQ=255)  gcggATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGata                                                                <  1:354958/81‑1 (MQ=255)    ggATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGatat                                                               >  1:354983/1‑80 (MQ=255)    ggATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGAtatt                                                              <  2:354959/81‑1 (MQ=255)      aTAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGAtattat                                                            <  1:354960/81‑1 (MQ=255)           gctgcGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACAt                                                       >  1:354992/1‑81 (MQ=255)             tgcGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACAttt                                                     >  1:354994/1‑81 (MQ=255)              gcGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACAttt                                                     >  2:354997/1‑80 (MQ=255)               cGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTc                                                    <  2:354961/80‑1 (MQ=255)                 aaaaGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATg                                                 <  2:354962/81‑1 (MQ=255)                  aaaGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGa                                                >  2:354996/1‑81 (MQ=255)                       agCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGactact                                           <  1:354963/81‑1 (MQ=255)                                ttATGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACt                                   >  1:354985/1‑80 (MQ=255)                                  aTGTAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTaa                                 <  2:354964/80‑1 (MQ=255)                                     tAACATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAAt                              >  2:354989/1‑80 (MQ=255)                                      aaCATAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAAtt                             >  1:354990/1‑80 (MQ=255)                                         aTAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACa                         >  2:354998/1‑81 (MQ=255)                                         aTAATGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACa                         >  1:354991/1‑81 (MQ=255)                                           aaTGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACAt                        >  2:354988/1‑80 (MQ=255)                                             tGCGACCAATAATCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGAc                     <  1:354965/81‑1 (MQ=255)                                                      taatCGTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGt            <  2:354966/81‑1 (MQ=255)                                                           gTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTccc       >  1:354968/1‑81 (MQ=255)                                                           gTAATGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTcc        <  2:354967/80‑1 (MQ=255)                                                               tGAATATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGa   <  2:354969/81‑1 (MQ=255)                                                                 aaTATGAGGAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGaa  >  1:354999/1‑80 (MQ=255)                                                                         |                                                                        GGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAA  >  NC_000913/1942542‑1942685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑