Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,990,813	T→A	V378E (GTG→GAG)	tyrP →	tyrosine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,990,813	0	T	A	96.5%	96.5 / ‑6.1	29	V378E (GTG→GAG)	tyrP	tyrosine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (7/21);  minor base G (1/0);  total (8/21)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGG  >  NC_000913/1990742‑1990888                                                                        |                                                                            gTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGtt                                                                    >  2:363489/1‑81 (MQ=255)    gACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTtc                                                                  <  2:363446/80‑1 (MQ=255)    gACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTtc                                                                  <  2:363445/80‑1 (MQ=255)     aCCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTtct                                                                 <  1:363447/80‑1 (MQ=255)      ccTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTctct                                                               >  1:363497/1‑81 (MQ=255)        tGGCAAAGCAGAAAGCACAATCCTCCGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGGGGTGGTGTTTCTCtgt                                                             >  2:363488/1‑81 (MQ=255)           cAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGt                                                          <  2:363448/81‑1 (MQ=255)            aaaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTa                                                         >  1:363490/1‑81 (MQ=255)             aaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTAt                                                        <  2:363450/81‑1 (MQ=255)             aaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTAt                                                        <  2:363449/81‑1 (MQ=255)               gCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTg                                                      <  2:363451/81‑1 (MQ=255)                 aGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCt                                                    >  2:363493/1‑81 (MQ=255)                       cacaATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGAtt                                              <  1:363452/81‑1 (MQ=255)                       cacaATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGAtt                                              <  1:363453/81‑1 (MQ=255)                              cTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTg                                        <  1:363455/80‑1 (MQ=255)                              cTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGc                                       <  2:363454/81‑1 (MQ=255)                                 aGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAAt                                    <  1:363456/81‑1 (MQ=255)                                  ggCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAAtt                                   <  1:363457/81‑1 (MQ=255)                                  ggCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAAtt                                   >  1:363492/1‑81 (MQ=255)                                   gCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAAttt                                  <  2:363458/81‑1 (MQ=255)                                          aCCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGAtt                            <  2:363459/80‑1 (MQ=255)                                           ccGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGc                          <  1:363460/81‑1 (MQ=255)                                             gggTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCgg                        <  2:363461/81‑1 (MQ=255)                                                    aGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgtt                 <  1:363462/81‑1 (MQ=255)                                                       tggtCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttgtt              <  2:363463/81‑1 (MQ=255)                                                        ggtCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTa             >  1:363496/1‑81 (MQ=255)                                                          tcgtcCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTAcc           <  2:363464/81‑1 (MQ=255)                                                            gtcCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCa          <  1:363465/80‑1 (MQ=255)                                                                   gcTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTggg  >  1:363502/1‑81 (MQ=255)                                                                        |                                                                            GTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGG  >  NC_000913/1990742‑1990888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑