Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,055,073	G→A	G5S (GGC→AGC)	amn →	AMP nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,055,073	0	G	A	100.0%	69.5 / NA	20	G5S (GGC→AGC)	amn	AMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/11);  total (9/11)

TTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGC  >  NC_000913/2054998‑2055147                                                                            |                                                                           ttGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCt                                                                         <  2:374299/79‑1 (MQ=255)  tGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTcc                                                                       <  1:374300/80‑1 (MQ=255)   gTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCgg                                                                     >  2:374322/1‑81 (MQ=255)   gTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCg                                                                      <  1:374301/80‑1 (MQ=255)    tttCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCGGt                                                                    >  1:374324/1‑81 (MQ=255)              cTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCGGTCTGACCCCa                                                           <  2:374302/80‑1 (MQ=255)                 tgACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCGGTCTGACCCCAGCt                                                        <  1:494799/80‑1 (MQ=255)                 tgACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCGGTCTGACCCCAGCTc                                                       <  1:374303/81‑1 (MQ=255)                     aTACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCGGTCTGACCCCAGCTCAGGc                                                   <  2:374304/81‑1 (MQ=255)                      tACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCGGTCTGACCCCAGCTCAGGCa                                                  >  2:374328/1‑81 (MQ=255)                            cGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCGGTCTGACCCCAGCTCAGGCACTGGa                                             >  1:374323/1‑80 (MQ=255)                                     ggTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGTTTCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAc                                   >  2:374333/1‑81 (MQ=255)                                     ggTAATTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAc                                   <  1:374305/81‑1 (MQ=255)                                         aTTGTATGGAACAGGAGACACACATGAATAATAAGAGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACgcgc                               >  1:374326/1‑81 (MQ=255)                                                         gaCACACATGAATAATAAGAGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGt               <  1:374306/81‑1 (MQ=255)                                                           cacacaTGAATAATAAGAGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAg             >  2:374330/1‑81 (MQ=255)                                                              acaTGAATAATAAGAGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCg          >  2:374334/1‑81 (MQ=255)                                                                  gAATAATAAGAGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCAt       >  1:374325/1‑80 (MQ=255)                                                                    ataataAGAGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTAc    <  1:374307/81‑1 (MQ=255)                                                                       ataaGAGCTCCGGTCTGACCCCAGCTCAGGCACTAGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGc  <  2:374308/80‑1 (MQ=255)                                                                            |                                                                           TTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGC  >  NC_000913/2054998‑2055147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑